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在物理模拟的户外条件下用CCAP19/18生产β-胡萝卜素。

Production of β-carotene with CCAP19/18 at physically simulated outdoor conditions.

作者信息

Wolf Lara, Cummings Thomas, Müller Katharina, Reppke Manfred, Volkmar Marianne, Weuster-Botz Dirk

机构信息

Institute of Biochemical Engineering Technical University of Munich Garching Germany.

出版信息

Eng Life Sci. 2020 Sep 13;21(3-4):115-125. doi: 10.1002/elsc.202000044. eCollection 2021 Mar.

DOI:10.1002/elsc.202000044
PMID:33716611
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7923581/
Abstract

Batch growth and β-carotene production of CCAP19/18 was investigated in flat-plate gas-lift photobioreactors with a light path of 2 cm, operated in physically simulated outdoor conditions. CCAP19/18 showed robust growth with respect to pH 8.0-9.0 and 15-35°C at increasing salinity, simulating the evaporation of open photobioreactors. The highest β-carotene concentration of 25 mg L (3 mg g ) was observed in batch processes at pH 8.5, 15-30°C and increasing salinity up to 110 g L, simulating a typical Mediterranean summer climate. Intracellular β-carotene accumulation of CCAP19/18 was shown to be independent of light availability, although nutrient limitation (KHPO, MgSO, and/or ammonium ferric citrate) seems to enable stable β-carotene content in the algal cells despite increasing cell densities in the photobioreactor. Fully controlled, lab-scale photobioreactors simulating typical climate conditions of any region of interest are valuable tools for enabling a realistic characterization of microalgae on a laboratory scale, for production processes projected in open photobioreactor systems (e.g. thin-layer cascade photobioreactors).

摘要

在具有2厘米光程的平板气升式光生物反应器中，研究了CCAP19/18在物理模拟户外条件下的分批生长和β-胡萝卜素生产情况。CCAP19/18在pH值为8.0 - 9.0、温度为15 - 35°C且盐度增加的条件下表现出强劲生长，模拟了开放式光生物反应器的蒸发情况。在pH值为8.5、温度为15 - 30°C且盐度增加至110克/升的分批过程中，观察到最高β-胡萝卜素浓度为25毫克/升（3毫克/克），模拟了典型的地中海夏季气候。尽管营养限制（KH₂PO₄、MgSO₄和/或柠檬酸铁铵）似乎能使光生物反应器中细胞密度增加时藻类细胞中的β-胡萝卜素含量保持稳定，但CCAP19/18细胞内β-胡萝卜素的积累显示与光照可用性无关。完全可控的实验室规模光生物反应器模拟任何感兴趣区域的典型气候条件，是在实验室规模上对微藻进行实际表征的宝贵工具，可用于开放式光生物反应器系统（如薄层级联光生物反应器）中的生产过程。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/da8e599cd136/ELSC-21-115-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/e2144d0983ea/ELSC-21-115-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/20112136266c/ELSC-21-115-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/0013294243a1/ELSC-21-115-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/f4fdb1810041/ELSC-21-115-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/de379b5ca3d7/ELSC-21-115-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/da8e599cd136/ELSC-21-115-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/e2144d0983ea/ELSC-21-115-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/20112136266c/ELSC-21-115-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/0013294243a1/ELSC-21-115-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/f4fdb1810041/ELSC-21-115-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/de379b5ca3d7/ELSC-21-115-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/edb7/7923581/da8e599cd136/ELSC-21-115-g005.jpg

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