Stanley P
Department of Cell Biology, Albert Einstein College of Medicine, Bronx, New York 10461.
Mol Cell Biol. 1989 Feb;9(2):377-83. doi: 10.1128/mcb.9.2.377-383.1989.
The production of glycoproteins with carbohydrates of defined structure and minimal heterogeneity is important for functional studies of mammalian carbohydrates. To facilitate such studies, several Chinese hamster ovary mutants that carry between two and four glycosylation mutations were developed. All of the lines grew readily in culture despite the drastic simplification of their surface carbohydrates. Therefore, both endogenous glycoproteins and those introduced by transfection can be obtained with specifically tailored carbohydrates. The lectin resistance properties of the mutants showed that each line expresses a novel array of cell surface carbohydrates useful for identifying specific roles for carbohydrates in cellular interactions. In addition, they showed that the epistatic relationships among different glycosylation mutations are not entirely predictable, providing insight into the complexity of the carbohydrate structures at the Chinese hamster ovary cell surface.
生产具有确定结构且异质性最小的碳水化合物的糖蛋白，对于哺乳动物碳水化合物的功能研究很重要。为便于此类研究，开发了几种携带两到四个糖基化突变的中国仓鼠卵巢突变体。尽管其表面碳水化合物大幅简化，但所有这些细胞系在培养中都能轻松生长。因此，可以获得具有特定定制碳水化合物的内源性糖蛋白和通过转染引入的糖蛋白。突变体的凝集素抗性特性表明，每个细胞系都表达了一系列新型的细胞表面碳水化合物，可用于确定碳水化合物在细胞相互作用中的特定作用。此外，这些特性还表明，不同糖基化突变之间的上位关系并非完全可预测，这为了解中国仓鼠卵巢细胞表面碳水化合物结构的复杂性提供了线索。
