细胞表面唾液酸残基在麦胚凝集素与亲本及麦胚凝集素抗性中国仓鼠卵巢细胞结合中的差异参与情况。
Differential involvement of cell surface sialic acid residues in wheat germ agglutinin binding to parental and wheat germ agglutinin-resistant Chinese hamster ovary cells.
作者信息
Stanley P, Sudo T, Carver J P
出版信息
J Cell Biol. 1980 Apr;85(1):60-9. doi: 10.1083/jcb.85.1.60.
Abstract
Two Chinese hamster ovary (CHO) cell mutants selected for resistance to wheat germ agglutinin (WGA) have been shown to exhibit defective sialylation of membrane glycoproteins and a membrane glycolipid, GM3. The mutants (termed WgaRII and WgaRIII) have been previously shown to belong to different genetic complementation groups and to exhibit different WGA-binding abilities. These mutants and a WGA-resistant CHO cell mutant termed WgaRI (which also possesses a surface sialylation defect arising from a deficient N-acetylglucosaminyltransferase activity), have enabled us to investigate the role of sialic acid in WGA binding at the cell surface. Scatchard plots of the binding of 125I-WGA (1 ng/ml to 1 mg/ml) to parental and WgaR CHO cells before and after a brief treatment with neuraminidase provide evidence for several different groups of sialic acid residues at the CHO cell surface which may be distinquished by their differential involvement in WGA binding to CHO cells.
摘要
已证实，通过筛选获得的两种对麦胚凝集素（WGA）具有抗性的中国仓鼠卵巢（CHO）细胞突变体，其膜糖蛋白和一种膜糖脂GM3的唾液酸化存在缺陷。这些突变体（称为WgaRII和WgaRIII）先前已被证明属于不同的遗传互补组，并表现出不同的WGA结合能力。这些突变体以及另一种称为WgaRI的对WGA具有抗性的CHO细胞突变体（其表面唾液酸化缺陷也是由N - 乙酰葡糖胺基转移酶活性不足引起的），使我们能够研究唾液酸在细胞表面WGA结合中的作用。在用神经氨酸酶进行短暂处理前后，125I - WGA（浓度从1 ng/ml到1 mg/ml）与亲本CHO细胞和WgaR CHO细胞结合的Scatchard图，为CHO细胞表面存在几组不同的唾液酸残基提供了证据，这些残基可能因其在WGA与CHO细胞结合中的不同参与情况而有所区别。
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本文引用的文献
1
Identification of a tumor-specific determinant on neoplastic cell surfaces.肿瘤细胞表面肿瘤特异性决定簇的鉴定。
Proc Natl Acad Sci U S A. 1967 Feb;57(2):359-66. doi: 10.1073/pnas.57.2.359.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
A SIMPLE AND ACCURATE MICROMETHOD FOR QUANTITATIVE DETERMINATION OF GANGLIOSIDE PATTERNS.一种用于定量测定神经节苷脂模式的简单准确的微量方法。
Life Sci (1962). 1964 Nov;3:1227-33. doi: 10.1016/0024-3205(64)90040-2.
4
Methods for the quantitative estimation of N-acetylneuraminic acid and their application to hydrolysates of sialomucoids.N-乙酰神经氨酸的定量测定方法及其在唾液酸粘蛋白水解产物中的应用。
Biochem J. 1961 Nov;81(2):384-92. doi: 10.1042/bj0810384.
5
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.唾液酸的定量测定。II. 比色间苯二酚 - 盐酸法。
Biochim Biophys Acta. 1957 Jun;24(3):604-11. doi: 10.1016/0006-3002(57)90254-8.
6
Analytical separation of nonlipid water soluble substances and gangliosides from other lipids by dextran gel column chromatography.通过葡聚糖凝胶柱色谱法从其他脂质中分析分离非脂质水溶性物质和神经节苷脂。
J Am Oil Chem Soc. 1965 Nov;42(11):913-9. doi: 10.1007/BF02632444.
7
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
8
Glycosphingolipids of wild-type and mutant lectin-resistant Chinese hamster ovarian cells.
Biochem Biophys Res Commun. 1974 Feb 27;56(4):1010-6. doi: 10.1016/s0006-291x(74)80289-5.
9
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
Eur J Biochem. 1974 Jul 1;46(1):83-8. doi: 10.1111/j.1432-1033.1974.tb03599.x.
10
External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes.人红细胞糖脂和糖蛋白中细胞表面半乳糖和氨基半乳糖的外部标记
J Biol Chem. 1973 Jun 25;248(12):4311-7.
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