Clayton C E, Fox J A
Rockefeller University, New York, NY 10021.
Mol Biochem Parasitol. 1989 Feb;33(1):73-9. doi: 10.1016/0166-6851(89)90044-3.
Methods were developed for in vivo labelling of trypanosome proteins with inorganic phosphate and the labelling of fructose bisphosphate aldolase and variant surface glycoprotein was investigated. It was found that the large pool size of phosphate in trypanosomes precludes detailed kinetic analyses. Aldolase contains low levels of phosphoserine but the function of this phosphorylation has yet to be determined.
已开发出用无机磷酸盐对锥虫蛋白质进行体内标记的方法，并对果糖二磷酸醛缩酶和可变表面糖蛋白的标记进行了研究。结果发现，锥虫中磷酸盐的大量储备妨碍了详细的动力学分析。醛缩酶含有低水平的磷酸丝氨酸，但其磷酸化的功能尚未确定。
