Clayton C E
Rockefeller University, New York, NY 10021-6399.
Mol Biochem Parasitol. 1988 Feb;28(1):43-6. doi: 10.1016/0166-6851(88)90178-8.
Procyclic Trypanosoma brucei were labelled with [35S]methionine, then incubated for up to 2 days in medium containing excess unlabelled methionine under conditions suitable for exponential growth. Neither fructose bisphosphate aldolase, a glycosomal enzyme, nor any of the major labelled proteins showed any detectable degradation.
将前循环型布氏锥虫用[35S]甲硫氨酸标记,然后在含有过量未标记甲硫氨酸的培养基中,于适合指数生长的条件下培养长达2天。糖酵解酶果糖二磷酸醛缩酶和任何主要的标记蛋白均未显示出任何可检测到的降解。