果糖二磷酸醛缩酶导入布氏锥虫的糖体中。
Import of fructose bisphosphate aldolase into the glycosomes of Trypanosoma brucei.
作者信息
Clayton C E
机构信息
Rockefeller University, New York 10021-6399.
出版信息
J Cell Biol. 1987 Dec;105(6 Pt 1):2649-54. doi: 10.1083/jcb.105.6.2649.
Abstract
The glycolytic enzymes of Trypanosomatids are compartmentalized within peroxisome-like microbodies called glycosomes. Fructose bisphosphate aldolase is synthesized on free polysomes and imported into glycosomes within 5 min. Peptide mapping reveals no primary structural differences between the in vivo-synthesized protein and that made in vitro from a synthetic template. However, native aldolase from glycosomes is partially protease resistant, whereas the in vitro translation product is not. Pulse-chase results indicate that aldolase in bloodstream trypanosomes has a much longer half-life than in the procyclic tsetse fly form.
摘要
锥虫的糖酵解酶被分隔在称为糖体的过氧化物酶体样微体中。果糖二磷酸醛缩酶在游离多核糖体上合成，并在5分钟内导入糖体。肽图分析表明，体内合成的蛋白质与体外从合成模板合成的蛋白质在一级结构上没有差异。然而，来自糖体的天然醛缩酶对蛋白酶有部分抗性，而体外翻译产物则没有。脉冲追踪结果表明，血液中的锥虫中的醛缩酶半衰期比采采蝇前循环型中的长得多。
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本文引用的文献
1
Biosynthesis of peroxisomal enzymes in the methylotrophic yeast Hansenula polymorpha.甲醇营养型酵母异常汉逊酵母中过氧化物酶体酶的生物合成。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6872-6. doi: 10.1073/pnas.80.22.6872.
2
Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids.用于核酸偶联和杂交的重氮化芳胺纤维素纸。
Nucleic Acids Res. 1982 Mar 11;10(5):1799-810. doi: 10.1093/nar/10.5.1799.
3
Oligomerization of malate synthase during glyoxysome biosynthesis.乙醛酸循环体生物合成过程中苹果酸合酶的寡聚化
Arch Biochem Biophys. 1983 Jun;223(2):629-38. doi: 10.1016/0003-9861(83)90627-6.
4
Malate synthase: aggregation, deaggregation, and binding of phospholipids.苹果酸合酶：聚集、解聚及磷脂结合
Arch Biochem Biophys. 1983 Jun;223(2):618-28. doi: 10.1016/0003-9861(83)90626-4.
5
Biosynthesis and intracellular transport of enzymes of peroxisomal beta-oxidation.过氧化物酶体β-氧化酶的生物合成与细胞内运输
J Biol Chem. 1984 May 25;259(10):6397-402.
6
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J Cell Biol. 1984 Apr;98(4):1178-84. doi: 10.1083/jcb.98.4.1178.
7
Stability of quaternary structure and mode of dissociation of fructosediphosphate aldolase isoenzymes.
Biochemistry. 1984 Oct 23;23(22):5257-61. doi: 10.1021/bi00317a025.
8
Simultaneous purification of hexokinase, class-I fructose-bisphosphate aldolase, triosephosphate isomerase and phosphoglycerate kinase from Trypanosoma brucei.从布氏锥虫中同时纯化己糖激酶、I类果糖二磷酸醛缩酶、磷酸丙糖异构酶和磷酸甘油酸激酶。
Eur J Biochem. 1984 Nov 2;144(3):475-83. doi: 10.1111/j.1432-1033.1984.tb08490.x.
9
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Exp Parasitol. 1980 Oct;50(2):240-50. doi: 10.1016/0014-4894(80)90025-9.
10
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J Biol Chem. 1984 Jul 10;259(13):8485-93.
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