用于检测马巴贝斯虫的重复DNA探针。

Repetitive DNA probes for the detection of Babesia equi.

作者信息

Posnett E S, Ambrosio R E

机构信息

Molecular Biology Section, Veterinary Research Institute, Onderstepoort, South Africa.

出版信息

Mol Biochem Parasitol. 1989 Apr;34(1):75-8. doi: 10.1016/0166-6851(89)90021-2.

DOI:10.1016/0166-6851(89)90021-2

PMID:2710167

Abstract

This report describes DNA probes for the identification of Babesia equi. A genomic library of B. equi was constructed in pUC13. Several clones were identified that hybridized strongly to B. equi DNA. Clone pBE33 hybridized specifically to B. equi DNA and did not hybridize to horse DNA nor to DNA from Babesia caballi, Babesia bovis or Babesia bigemina. Two subclones of pBE33 (pSB20 and pEH21) containing B. equi repetitive sequences, could detect 0.49 ng and 0.97 ng B. equi DNA, respectively.

摘要

本报告描述了用于鉴定马巴贝斯虫的DNA探针。构建了马巴贝斯虫在pUC13中的基因组文库。鉴定出了几个与马巴贝斯虫DNA强烈杂交的克隆。克隆pBE33与马巴贝斯虫DNA特异性杂交，不与马DNA杂交，也不与来自驽巴贝斯虫、牛巴贝斯虫或双芽巴贝斯虫的DNA杂交。包含马巴贝斯虫重复序列的pBE33的两个亚克隆（pSB20和pEH21）分别能检测到0.49 ng和0.97 ng的马巴贝斯虫DNA。