菲律宾马中伊氏锥虫和马媾疫锥虫的血清学和分子检测。
Serological and molecular detection of Theileria equi and Babesia caballi in Philippine horses.
机构信息
College of Veterinary Medicine at Barili Campus and College of Arts and Sciences at Main Campus, Cebu Technological University, Corner M.J. Cuenco Ave. and R. Palma St., Cebu City 6000, Philippines; Department of Research, Gullas College of Medicine, University of the Visayas, Cebu, Philippines; College of Science, University of the Philippines Cebu, Gorordo Avenue, Lahug, Cebu City 6000, Philippines.
College of Science, University of the Philippines Cebu, Gorordo Avenue, Lahug, Cebu City 6000, Philippines; National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
出版信息
Ticks Tick Borne Dis. 2018 Jul;9(5):1125-1128. doi: 10.1016/j.ttbdis.2018.04.010. Epub 2018 Apr 18.
Theileria equi and Babesia caballi are tick-borne protozoan parasites that can cause anemia in horses. In the Philippines, serological detection of these parasites has only been reported in the Northern area (Luzon). In this study, 105 horses from Cebu and Bohol, Philippines were tested using peripheral blood smear examination (PBSE), immunochromatographic test (ICT) strips, and PCR. Clinical history, presenting clinical signs and complete blood count were obtained. Results revealed that although all horses were negative using PBSE, 23 (21.9%) were positive (12 for T. equi, and 11 for B. caballi) using ICT. PCR revealed 26 and 2 horses positive for T. equi and B. caballi, respectively. All positive horses showed no clinical signs. Partial DNA sequences of representative amplicons were found 100% identical to GenBank registered T. equi and B. caballi sequences. Statistical analyses revealed that location was found associated with T. equi PCR positivity and B. caballi seropositivity. This study documents the first serological detection of T. equi and B. caballi in horses in the southern area of the Philippines, and their first molecular detection and characterization in the country.
马媾疫锥虫和马巴贝斯虫是通过蜱传播的原生动物寄生虫，可引起马贫血。在菲律宾，这些寄生虫的血清学检测仅在北部地区（吕宋岛）有报道。在这项研究中，使用外周血涂片检查（PBSE）、免疫层析试验（ICT）条和 PCR 对来自菲律宾宿务和薄荷岛的 105 匹马进行了检测。获得了临床病史、临床表现和全血细胞计数。结果表明，尽管所有马匹的 PBSE 检测均为阴性，但使用 ICT 检测时，有 23 匹马（12 匹为 T. equi，11 匹为 B. caballi）为阳性。PCR 显示分别有 26 匹马和 2 匹马对 T. equi 和 B. caballi 呈阳性。所有阳性马匹均无症状。代表性扩增子的部分 DNA 序列与 GenBank 注册的 T. equi 和 B. caballi 序列完全一致。统计分析表明，地理位置与 T. equi PCR 阳性和 B. caballi 血清阳性有关。本研究首次在菲律宾南部地区的马中检测到 T. equi 和 B. caballi 的血清学，并首次在该国对其进行了分子检测和鉴定。
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