Greenfield Melinda, Gómez-Jiménez María I, Ortiz Viviana, Vega Fernando E, Kramer Matthew, Parsa Soroush
Entomology, International Center for Tropical Agriculture (CIAT), km. 17, Recta Cali-Palmira, Colombia.
Sustainable Perennial Crops Laboratory, United States Department of Agriculture, Agricultural Research Service, Building 001, Beltsville, MD 20705, USA.
Biol Control. 2016 Apr;95:40-48. doi: 10.1016/j.biocontrol.2016.01.002.
We investigated the fungal entomopathogens and to determine if endophytic colonization could be achieved in cassava. An inoculation method based on drenching the soil around cassava stem cuttings using conidial suspensions resulted in endophytic colonization of cassava roots by both entomopathogens, though neither was found in the leaves or stems of the treated cassava plants. Both fungal entomopathogens were detected more often in the proximal end of the root than in the distal end. Colonization levels of were higher when plants were sampled at 7-9 days post-inoculation (84%) compared to 47-49 days post-inoculation (40%). In contrast, the colonization levels of remained constant from 7-9 days post-inoculation (80%) to 47-49 days post-inoculation (80%), which suggests is better able to persist in the soil, or as an endophyte in cassava roots over time. Differences in colonization success and plant growth were found among the fungal entomopathogen treatments.
我们研究了真菌性昆虫病原体,并确定木薯是否能够实现内生定殖。一种基于使用分生孢子悬浮液浇灌木薯茎切段周围土壤的接种方法,使得两种昆虫病原体都在木薯根中实现了内生定殖,不过在经过处理的木薯植株的叶片或茎中均未发现这两种病原体。两种真菌性昆虫病原体在根的近端被检测到的频率均高于远端。与接种后47 - 49天(40%)相比,在接种后7 - 9天对植株进行采样时,[某种病原体]的定殖水平更高(84%)。相比之下,[另一种病原体]的定殖水平从接种后7 - 9天(80%)到接种后47 - 49天(80%)保持恒定,这表明[该病原体]能够更好地长期存在于土壤中,或者作为木薯根中的内生菌存在。在真菌性昆虫病原体处理之间发现了定殖成功率和植株生长方面的差异。