Tokarski Alessandra, Ribeiro Luana Karolline, Alves Guilherme Lopes, Azevedo Fabiana Lustosa, Nardi Cristiane, do Prado Ribeiro Leandro
State Univ of Midwest of Paraná (UNICENTRO), Guarapuava, Paraná, Brazil.
Research Center for Family Agriculture, Agricultural Research and Rural Extension Company of Santa Catarina (CEPAF/EPAGRI), Chapecó, Santa Catarina, Brazil.
Neotrop Entomol. 2025 Jan 23;54(1):29. doi: 10.1007/s13744-024-01237-3.
To understand the interactions of entomopathogenic fungi with forage plants and their influence on associated herbivorous, we evaluated the influence of endophytic colonization with three isolates (CEPAF_ENT 25, CEPAF_ENT 27, and IBCB 425) of Metarhizium anisopliae on Cynodon dactylon, regarding the biological and behavioral aspects of Collaria scenica, an emerging sucking pest in pastoral systems in Brazil. The application of suspensions at the base of plant (drench) was effective in promoting endophytic colonization, especially in the roots, with emphasis on isolates CEPAF_ENT25 and CEPAF_ENT27. Despite the significant reduction in damage caused by C. scenica observed in plants colonized with isolate CEPAF_ENT 27 in a choice test, our results indicated a limited influence of the isolates tested on the host selection behavior and in C. scenica development, including parameters of the fertility life table. The cluster analysis, however, indicated a significant difference between all isolates tested in terms of the chemical-bromatological composition and the levels of photosynthetic pigments in inoculated plants. The biplot analysis indicated that isolate CEPAF-ENT27 (most promising) showed a positive correlation with nitrogen, potassium, calcium, boron, copper, and zinc levels, as well as with chlorophyll a and b levels. Additionally, foliar magnesium levels were significantly higher in all isolates tested. Improvements in plant nutrition may increase tolerance in infested plants through compensation mechanisms, contributing to more resilient pastoral systems.
为了解昆虫病原真菌与饲草植物的相互作用及其对相关食草动物的影响，我们评估了用三种绿僵菌分离株（CEPAF_ENT 25、CEPAF_ENT 27和IBCB 425）对狗牙根进行内生定殖，对巴西牧区系统中一种新出现的刺吸式害虫——场景科拉叶蝉生物学和行为方面的影响。在植株基部施用悬浮液（灌根）有效地促进了内生定殖，尤其是在根部，其中CEPAF_ENT25和CEPAF_ENT27分离株尤为突出。尽管在选择试验中观察到，用CEPAF_ENT 27分离株定殖的植株上场景科拉叶蝉造成的损害显著减少，但我们的结果表明，所测试的分离株对寄主选择行为和场景科拉叶蝉发育（包括繁殖力生命表参数）的影响有限。然而，聚类分析表明，在所测试的所有分离株之间，接种植株的化学-溴成分和光合色素水平存在显著差异。双标图分析表明，CEPAF-ENT27分离株（最具前景）与氮、钾、钙、硼、铜和锌水平以及叶绿素a和b水平呈正相关。此外，所有测试分离株的叶片镁含量均显著较高。植物营养的改善可能通过补偿机制提高受侵染植物的耐受性，有助于建立更具韧性的牧区系统。
