Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea; Department of Marine Biotechnology, University of Science and Technology, Daejeon, Republic of Korea.
Korea Institute of Ocean Science and Technology, Ansan, Republic of Korea; Department of Microbiology, Chungbuk National University, Republic of Korea.
Bioresour Technol. 2016 Jul;211:792-6. doi: 10.1016/j.biortech.2016.04.038. Epub 2016 Apr 12.
Acetogens have often been observed to be inhibited by CO above an inhibition threshold concentration. In this study, a two-stage culture consisting of carboxydotrophic archaea and homoacetogenic bacteria is found to be effective in converting industrial waste gas derived from a steel mill process. In the first stage, Thermococcus onnurineus could grow on the Linz-Donawitz converter gas (LDG) containing ca. 56% CO as a sole energy source, converting the CO into H2 and CO2. Then, in the second stage, Thermoanaerobacter kivui could grow on the off-gas from the first stage culture, consuming the H2 and CO in the off-gas completely and producing acetate as a main product. T. kivui alone could not grow on the LDG gas. This work represents the first demonstration of acetate production using steel mill waste gas by a two-stage culture of carboxydotrophic hydrogenogenic microbes and homoacetogenic bacteria.
产乙酸菌通常会被 CO 抑制，其抑制阈值浓度因种属不同而有所差异。在本研究中，我们发现两段式培养（由产碳氢化合物的古菌和同型产乙酸菌组成）可有效地将钢厂工艺产生的工业废气转化为有用物质。在第一阶段，Thermococcus onnurineus 可以利用林茨-多纳维茨转炉煤气（LDG）作为唯一的能源生长，LDG 中含有约 56%的 CO，在此过程中，CO 被转化为 H2 和 CO2。然后，在第二阶段，Thermoanaerobacter kivui 可以利用第一阶段培养的尾气生长，完全消耗尾气中的 H2 和 CO，并产生乙酸作为主要产物。单独的 T. kivui 不能利用 LDG 气体生长。这项工作首次证明了利用两段式产碳氢化合物的产氢微生物和同型产乙酸菌培养法，从钢厂废气中生产乙酸。
