Sulfaro S, Volpe R, Miniutti C, Carbone A, Barzan L, Comoretto R
Division of Pathology, Centro di Riferimento Oncologico, Aviano, Italy.
Pathol Res Pract. 1989 Feb;184(2):248-54. doi: 10.1016/S0344-0338(89)80127-X.
A procedure of pathological analysis of laryngopharyngeal specimens by whole organ sections in the horizontal plane was developed and evaluated. The use of a fast decalcifying and fixative solution allowed both an easier sectioning of the whole organ and thin (5-6 microns) histological whole organ sections of laryngo pharyngectomy specimens. The complete procedure required 5 to 10 days for each case. A preliminary examination of 39 surgical laryngopharyngectomy specimens with laryngeal or hypopharyngeal carcinomas showed that most of the normal structures were histologically identifiable on whole organ horizontal histological sections. The data of the local neoplastic spread related to the main sites of origin were in accordance with the results obtained by other studies using whole organ sections in the coronal or vertical planes. In conclusion, with this procedure it is possible to obtain, in a reasonably short time, thin histological horizontal whole organ sections allowing an adequate and complete assessment of the microscopic characteristics of the tumor and of the tridimensional relationships of the neoplasm with the host organ; moreover, by this procedure pathological data may be compared with preoperative CT or MR scans in patients with laryngeal and hypopharyngeal carcinomas.
开发并评估了一种通过水平平面的全器官切片对喉咽标本进行病理分析的方法。使用快速脱钙和固定液既便于对整个器官进行切片，又能获得喉咽切除术标本的薄（5 - 6微米）组织学全器官切片。每个病例的完整过程需要5到10天。对39例喉或下咽癌手术切除的喉咽标本进行初步检查发现，在全器官水平组织学切片上，大多数正常结构在组织学上是可识别的。与主要起源部位相关的局部肿瘤扩散数据与其他使用冠状面或垂直面全器官切片的研究结果一致。总之，通过该方法可以在合理的短时间内获得薄的组织学水平全器官切片，从而能够充分且完整地评估肿瘤的微观特征以及肿瘤与宿主器官的三维关系；此外，通过该方法，可将喉癌和下咽癌患者的病理数据与术前CT或MR扫描结果进行比较。
