Kho Elise A, Hugo Leon E, Lu Guangjin, Smith David D, Kay Brian H
QIMR Berghofer Medical Research Institute, 300 Herston Rd., Herston, Queensland 4006, Australia (
J Med Entomol. 2016 Jul;53(4):894-901. doi: 10.1093/jme/tjw029. Epub 2016 Apr 22.
In order to assess the broad-scale applicability of field releases of Wolbachia for the biological control of insect-transmitted diseases, we determined the relationship between the larval diet of Aedes aegypti L. mosquitoes infected with Wolbachia strains and their susceptibility to dengue virus (DENV) infection via intrathoracic injection and oral inoculation. Larvae were reared on diets that varied in the quantity of food which had the effect of modifying development time and adult body size. Wolbachia wMel infection was associated with highly significant reductions in dengue serotype 2 (DENV-2) infection rates of between 80 and 97.5% following intrathoracic injection of adults emerging from three diet levels. Reductions were 100% in two diet level treatments following oral inoculation. Similarly, wMelPop infection was associated with highly significant reductions in DENV-2 infection rates of between 95 and 100% for intrathoracic injection and 97.5 and 100% for oral inoculation across diet level treatments. Larval diet level had no significant effect on DENV-2 infection rates in the presence of Wolbachia infection in mosquitoes that were intrathoracically injected with the virus. This indicates that the effectiveness of Wolbachia on vector competence disruption within Ae. aegypti is unlikely to be compromised by variable larval nutrition in field settings.
为了评估释放沃尔巴克氏体进行昆虫传播疾病生物防治的广泛适用性，我们确定了感染沃尔巴克氏体菌株的埃及伊蚊幼虫饮食与其通过胸腔注射和口服接种感染登革病毒（DENV）易感性之间的关系。幼虫以食物量不同的饮食饲养，这些饮食对发育时间和成虫体型有影响。对于从三种饮食水平羽化出的成虫进行胸腔注射后，感染沃尔巴克氏体wMel与登革2型病毒（DENV-2）感染率显著降低80%至97.5%相关。口服接种后，两种饮食水平处理的感染率降低了100%。同样，在不同饮食水平处理中，感染wMelPop的蚊子经胸腔注射后DENV-2感染率显著降低95%至100%，经口服接种后降低97.5%至100%。在对经胸腔注射病毒的蚊子进行沃尔巴克氏体感染时，幼虫饮食水平对DENV-2感染率没有显著影响。这表明在野外环境中，沃尔巴克氏体对埃及伊蚊传播能力的破坏效果不太可能因幼虫营养的变化而受到影响。
