Beeravolu Naimisha, Khan Irfan, McKee Christina, Dinda Sumi, Thibodeau Bryan, Wilson George, Perez-Cruet Mick, Bahado-Singh Ray, Chaudhry G Rasul
Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA; OU-WB Institute for Stem Cell and Regenerative Medicine, Oakland University, Rochester, MI 48309, USA.
Department of Biological Sciences, Oakland University, Rochester, MI 48309, USA; OU-WB Institute for Stem Cell and Regenerative Medicine, Oakland University, Rochester, MI 48309, USA; Dr. Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Pakistan.
Stem Cell Res. 2016 May;16(3):696-711. doi: 10.1016/j.scr.2016.04.010. Epub 2016 Apr 13.
Human umbilical cord (hUC) blood and tissue are non-invasive sources of potential stem/progenitor cells with similar cell surface properties as bone marrow stromal cells (BMSCs). While they are limited in cord blood, they may be more abundant in hUC. However, the hUC is an anatomically complex organ and the potential of cells in various sites of the hUC has not been fully explored. We dissected the hUC into its discrete sites and isolated hUC cells from the cord placenta junction (CPJ), cord tissue (CT), and Wharton's jelly (WJ). Isolated cells displayed fibroblastoid morphology, and expressed CD29, CD44, CD73, CD90, and CD105, and showed evidence of differentiation into multiple lineages in vitro. They also expressed low levels of pluripotency genes, OCT4, NANOG, SOX2 and KLF4. Passaging markedly affected cell proliferation with concomitant decreases in the expression of pluripotency and other markers, and an increase in chondrogenic markers. Microarray analysis further revealed the differences in the gene expression of CPJ-, CT- and WJ-hUC cells. Five coding and five lncRNA genes were differentially expressed in low vs. high passage hUC cells. Only MAEL was expressed at high levels in both low and high passage CPJ-hUC cells. They displayed a greater proliferation limit and a higher degree of multi-lineage differentiation in vitro and warrant further investigation to determine their full differentiation capacity, and therapeutic and regenerative medicine potential.
人脐带（hUC）血和组织是潜在干细胞/祖细胞的非侵入性来源，其细胞表面特性与骨髓基质细胞（BMSCs）相似。虽然它们在脐带血中数量有限，但在hUC中可能更为丰富。然而，hUC是一个解剖结构复杂的器官，hUC各个部位细胞的潜能尚未得到充分探索。我们将hUC解剖成不同部位，并从脐带胎盘交界处（CPJ）、脐带组织（CT）和华通胶（WJ）中分离出hUC细胞。分离出的细胞呈现成纤维细胞样形态，表达CD29、CD44、CD73、CD90和CD105，并在体外显示出向多个谱系分化的证据。它们还低水平表达多能性基因OCT4、NANOG、SOX2和KLF4。传代显著影响细胞增殖，同时多能性及其他标志物的表达降低，而成软骨标志物表达增加。微阵列分析进一步揭示了CPJ-hUC细胞、CT-hUC细胞和WJ-hUC细胞基因表达的差异。在低代与高代hUC细胞中，五个编码基因和五个lncRNA基因差异表达。只有MAEL在低代和高代CPJ-hUC细胞中均高水平表达。它们在体外显示出更大的增殖极限和更高程度的多谱系分化，值得进一步研究以确定其完全分化能力以及治疗和再生医学潜能。
