Balzano Francesca, Bellu Emanuela, Basoli Valentina, Dei Giudici Silvia, Santaniello Sara, Cruciani Sara, Facchin Federica, Oggiano Annalisa, Capobianco Giampiero, Dessole Francesco, Ventura Carlo, Dessole Salvatore, Maioli Margherita
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy.
Department of Biomedical Sciences, University of Sassari, Viale San Pietro 43/B, 07100 Sassari, Italy; Laboratory of Molecular Biology and Stem Cell Engineering, National Institute of Biostructures and Biosystems - Eldor Lab, Innovation Accelerator, CNR, Via Piero Gobetti 101, 40129 Bologna, Italy.
Eur J Obstet Gynecol Reprod Biol. 2019 Mar;234:143-148. doi: 10.1016/j.ejogrb.2018.12.028. Epub 2019 Jan 22.
To study the molecular features of mesenchymal stem cells from Wharton Jelly (WJ-MSCs) of umbilical cord to predict their differentiation capacity.
Comparison of gene expression from mesenchymal stem cells of male and female umbilical cord SETTING: University hospital PATIENT (S): umbilical cords (n = 12, 6 males and 6 females) retrieved from spontaneous full-term vaginal delivery of healthy women INTERVENTION: we analyzed the expression of the stemness related genes C-MYC, OCT4, SOX2 and NANOG and of the epigenetic modulating gene DNA-methyltransferase 1 (DNMT1).
WJ-MSCs were isolated by standard procedures and immunophenotypically characterized. Gene expression analysis of stemness related genes and the epigenetic modulating gene DNMT1 were performed by real-time PCR RESULTS: expression of the OCT4 and DNMT1 genes was significantly higher in WJ- MSCs isolated from male subjects, as compared to MSCs isolated from female-derived WJ. The resulting higher expression of OCT4 and DNMT1 in WJ-MSCs from males as compared with female WJ-MSCs for the first time identifies a specific relationship between stemness genes, an epigenetic modulator, and gender differences.
our findings disclose novel biomedical implications in WJ-MSCs related to the sex of the donor, thus providing additional cues to exploit their regenerative potential in allogenic transplantation.
研究脐带华通氏胶间充质干细胞（WJ-MSCs）的分子特征，以预测其分化能力。
比较男性和女性脐带间充质干细胞的基因表达
大学医院
从健康女性自然足月阴道分娩获取的脐带（n = 12，6名男性和6名女性）
我们分析了干性相关基因C-MYC、OCT4、SOX2和NANOG以及表观遗传调节基因DNA甲基转移酶1（DNMT1）的表达。
通过标准程序分离WJ-MSCs并进行免疫表型特征分析。通过实时PCR对干性相关基因和表观遗传调节基因DNMT1进行基因表达分析
与从女性来源的WJ中分离的间充质干细胞相比，从男性受试者分离的WJ-MSCs中OCT4和DNMT1基因的表达显著更高。与女性WJ-MSCs相比，男性WJ-MSCs中OCT4和DNMT1的更高表达首次确定了干性基因、一种表观遗传调节剂和性别差异之间的特定关系。
我们的研究结果揭示了WJ-MSCs中与供体性别相关的新的生物医学意义，从而为在同种异体移植中利用其再生潜力提供了额外线索。
