Ranjbaran Hossein, Abediankenari Saeid, Mohammadi Masoud, Jafari Narjes, Khalilian Alireza, Rahmani Zahra, Momeninezhad Amiri Marzei, Ebrahimi Pirooz
Immunogenetics Research Center, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Biostatistics and Community Medicine, School of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Acta Med Iran. 2018 Jan;56(1):28-33.
Whartons jelly-derived mesenchymal stem cells (WJ-MSCs), have a high proliferation valency and they do not produce teratogen or carcinogen after subsequent transplantation. They are known as regenerative medicine. Thus more research is needed on the isolation and characterization of mesenchymal stem cells. In this experimental study, we obtained Wharton's jelly tissues from mothers during normal vaginal delivery, after obtaining their informed consent. Mesenchymal stem cells were isolated from cultured Whartons jelly, cultured, and were then examined for their proliferation, immunophenotypes, and differentiation capacities. The immunophenotypes of WJ-MSCs were analyzed by flow cytometry. Differentiation was performed resulting in osteogenic, chondrogenic and adipogenic cells. WJ-MSCs formed a homogenous monolayer of adherent spindle-shaped cells. Our results showed the high capacity of the proliferation of WJ-MSCs. Immunophenotyping further confirmed the purity of the isolated cells; their surface antigen expression showed the phenotypical properties like those of WJ-MSCs. The expanded cells were positive for CD 90, CD105, and CD44; they were negative for CD34 and HLA-DR surface markers. The cells had the adipocytic, osteocytic and chondrogenic differentiation capacity. The isolation and characterization of WJ-MSCs with high purity had been conducted, and the results were obtained in a short span. The present study has revealed the feasibility of the culture medium with high glucose and 15% FBS in isolation and proliferation of WJ-MSCs. When Wharton`s jelly pieces were put in the dry bottom of the flask, very effective separation of the MSCs was achieved.
源自脐带华通氏胶的间充质干细胞（WJ-MSCs）具有高增殖潜能，并且在后续移植后不会产生致畸物或致癌物。它们被视为再生医学的研究对象。因此，对于间充质干细胞的分离和特性鉴定仍需开展更多研究。在本实验研究中，我们在获得产妇知情同意后，于正常阴道分娩过程中获取了脐带华通氏胶组织。从培养的脐带华通氏胶中分离出间充质干细胞，进行培养，然后检测其增殖能力、免疫表型和分化能力。通过流式细胞术分析WJ-MSCs的免疫表型。诱导分化生成成骨细胞、软骨细胞和脂肪细胞。WJ-MSCs形成了由贴壁纺锤形细胞组成的均匀单层。我们的结果显示WJ-MSCs具有高增殖能力。免疫表型分析进一步证实了分离细胞的纯度；其表面抗原表达显示出与WJ-MSCs相似的表型特征。扩增后的细胞CD 90、CD105和CD44呈阳性；CD34和HLA-DR表面标志物呈阴性。这些细胞具有脂肪形成、骨形成和软骨形成的分化能力。我们成功进行了高纯度WJ-MSCs的分离和特性鉴定，且在短时间内获得了结果。本研究揭示了高糖和15%胎牛血清培养基在WJ-MSCs分离和增殖中的可行性。当将脐带华通氏胶碎片置于培养瓶底部干燥处时，能非常有效地分离出间充质干细胞。
