O'Shea C J, O'Doherty J V, Callanan J J, Doyle D, Thornton K, Sweeney T
School of Agriculture and Food Science , College of Life Sciences, University College Dublin , Belfield, Dublin 4 , Republic of Ireland.
School of Veterinary Medicine , College of Life Sciences, University College Dublin , Belfield, Dublin 4 , Republic of Ireland.
J Nutr Sci. 2016 Mar 28;5:e15. doi: 10.1017/jns.2016.4. eCollection 2016.
The algal polysaccharides laminarin (LAM) and fucoidan (FUC) have potent anti-inflammatory activities in the gastrointestinal tract. Our objective was to examine the impact of prior consumption of LAM and/or FUC on pathology and inflammation following a dextran sodium sulfate (DSS) challenge in pigs. Pigs (n 7/group) were assigned to one of five experimental groups for 56 d. From 49-55 d, distilled water or DSS was administered intragastrically. The experimental groups were: (1) basal diet + distilled water (control); (2) basal diet + DSS (DSS); (3) basal diet + FUC + DSS (FUC + DSS); (4) basal diet + LAM + DSS (LAM + DSS); and (5) basal diet + LAM + FUC + DSS (LAMFUC + DSS). The DSS group had decreased body-weight gain (P < 0·05) and serum xylose (P < 0·05), and increased proximal colon pathology score (P < 0·05), diarrhoeal score (P < 0·001) and colonic Enterobacteriaceae (P < 0·05) relative to the control group. The FUC + DSS (P < 0·01), LAM + DSS (P < 0·05) and LAMFUC + DSS (P < 0·05) groups had improved diarrhoeal score, and the LAMFUC + DSS (P < 0·05) group had improved body weight relative to the DSS group. The FUC + DSS group (P < 0·001), LAM + DSS group (P < 0·05) and LAMFUC + DSS group (P < 0·001) had lower IL-6 mRNA abundance relative to the DSS group. The LAM + DSS group had reduced Enterobacteriaceae in proximal colon digesta relative to the DSS group (P < 0·05). In conclusion, FUC or a combination of FUC and LAM improved body-weight loss, diarrhoeal scores and clinical variables associated with a DSS challenge in pigs, in tandem with a reduction in colonic IL-6 mRNA abundance.
藻类多糖海带多糖（LAM）和岩藻依聚糖（FUC）在胃肠道具有强大的抗炎活性。我们的目的是研究预先摄入LAM和/或FUC对猪在葡聚糖硫酸钠（DSS）攻击后病理和炎症的影响。将猪（每组n = 7）分配到五个实验组之一，为期56天。从第49 - 55天，通过胃内给予蒸馏水或DSS。实验组如下：（1）基础日粮 + 蒸馏水（对照组）；（2）基础日粮 + DSS（DSS组）；（3）基础日粮 + FUC + DSS（FUC + DSS组）；（4）基础日粮 + LAM + DSS（LAM + DSS组）；（5）基础日粮 + LAM + FUC + DSS（LAMFUC + DSS组）。与对照组相比，DSS组体重增加减少（P < 0.05），血清木糖降低（P < 0.05），近端结肠病理评分增加（P < 0.05），腹泻评分增加（P < 0.001），结肠肠杆菌科细菌数量增加（P < 0.05）。与DSS组相比，FUC + DSS组（P < 0.01）、LAM + DSS组（P < 0.05）和LAMFUC + DSS组（P < 0.05）腹泻评分有所改善，LAMFUC + DSS组（P < 0.05）体重有所改善。与DSS组相比，FUC + DSS组（P < 0.001）、LAM + DSS组（P < 0.05）和LAMFUC + DSS组（P < 0.001）白细胞介素-6（IL-6）mRNA丰度较低。与DSS组相比，LAM + DSS组近端结肠消化物中的肠杆菌科细菌数量减少（P < 0.05）。总之，FUC或FUC与LAM的组合改善了猪在DSS攻击后的体重减轻、腹泻评分和临床变量，同时结肠IL-6 mRNA丰度降低。
