Guo Guangzhen, Yang Weiguang, Fan Chaojie, Lan Ruixia, Gao Zhenhua, Gan Shangquan, Yu Haibin, Yin Fuquan, Wang Zhijing
College of Coastal Agriculture Science, Guangdong Ocean University, Zhanjiang, China.
The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Province, Department of Animal Science, Guangdong Ocean University, Zhanjiang, China.
Front Vet Sci. 2022 Oct 20;9:1007346. doi: 10.3389/fvets.2022.1007346. eCollection 2022.
This paper explores the effects of fucoidan on the frequency of diarrhea, colon morphology, colon antioxidant status, cytokine content, short-chain fatty acids, and microflora of cecal contents in early weaned lambs in order to provide a reference for the intestinal health of young ruminants. Fucoidan is a natural active polysaccharide extracted from kelp and other large brown algae. It has many biological effects, such as improving immunity, nourishing the stomach and intestines, and anti-tumor properties. This study investigated the effects of fucoidan supplementation in milk replacer on the large intestine's ability to act as an intestinal barrier in weaned lambs. With six duplicate pens and one lamb per pen, a total of 24 weaned lambs (average starting body weight of 7.32 ± 0.37 kg) were randomly assigned to one of four milk replacer treatments. Four concentrations of fucoidan supplementation (0, 0.1, 0.3, and 0.6% dry matter intake) were employed to investigate the effects of fucoidan on cecal fermentation and colon microbial organization. The test period lasted 37 days (1 week before the test and 1 month after the test), and lamb cecal contents and colon organization were collected for examination. In addition, the fecal status of all lambs was observed and recorded daily, allowing us to calculate the incidence of diarrhea in weaned lambs. The findings demonstrated that fucoidan may significantly increase the concentration of short-chain fatty acids (propionic acid and butyric acid) in the cecal digesta of weaned lambs. In weaned lambs, 16S rDNA testing showed that fucoidan at 0.3-0.6% (dry matter intake) was beneficial for boosting the variety of the intestinal bacteria and modifying the relative abundance of a few bacterial strains. In addition, fucoidan enhanced colon antioxidant and immune functions and decreased the diarrhea rate to relieve weaning stress. This result demonstrates that milk replacer supplementation with fucoidan contributes to the improvement in the large intestinal health of weaned lambs.
本文探讨了岩藻依聚糖对早期断奶羔羊腹泻频率、结肠形态、结肠抗氧化状态、细胞因子含量、短链脂肪酸以及盲肠内容物微生物群的影响，旨在为幼龄反刍动物的肠道健康提供参考。岩藻依聚糖是从海带和其他大型褐藻中提取的一种天然活性多糖。它具有多种生物学效应，如提高免疫力、滋养胃肠和抗肿瘤特性。本研究调查了在代乳粉中添加岩藻依聚糖对断奶羔羊大肠作为肠道屏障功能的影响。共有24只断奶羔羊（平均初始体重7.32±0.37千克），每6只重复饲养在一个围栏中，每围栏1只，随机分配到四种代乳粉处理之一。采用四种岩藻依聚糖添加浓度（0、0.1、0.3和0.6%干物质摄入量）来研究岩藻依聚糖对盲肠发酵和结肠微生物组成的影响。试验期持续37天（试验前1周和试验后1个月），收集羔羊盲肠内容物和结肠组织进行检测。此外，每天观察并记录所有羔羊的粪便状况，以便计算断奶羔羊的腹泻发生率。研究结果表明，岩藻依聚糖可能显著增加断奶羔羊盲肠消化物中短链脂肪酸（丙酸和丁酸）的浓度。在断奶羔羊中，16S rDNA检测表明，0.3 - 0.6%（干物质摄入量）的岩藻依聚糖有利于增加肠道细菌的种类并改变一些细菌菌株的相对丰度。此外，岩藻依聚糖增强了结肠的抗氧化和免疫功能，降低了腹泻率，以缓解断奶应激。这一结果表明，在代乳粉中添加岩藻依聚糖有助于改善断奶羔羊的大肠健康。
