一种快速的门诊检测方法能够高灵敏度和高特异性地检测犬类急性钩端螺旋体病。
A Rapid In-Clinic Test Detects Acute Leptospirosis in Dogs with High Sensitivity and Specificity.
作者信息
Kodjo Angeli, Calleja Christophe, Loenser Michael, Lin Dan, Lizer Joshua
机构信息
Laboratoire des Leptospires, VetAgro Sup, Campus Veterinaire de Lyon, 1 avenue Bourgelat, 69280 Marcy l'Etoile, France.
Zoetis Global Diagnostics, 100 Campus Drive, Florham Park, NJ 07932, USA.
出版信息
Biomed Res Int. 2016;2016:3760191. doi: 10.1155/2016/3760191. Epub 2016 Mar 24.
A rapid IgM-detection immunochromatographic test (WITNESS® Lepto, Zoetis) has recently become available to identify acute canine leptospirosis at the point of care. Diagnostic sensitivity and specificity of the test were evaluated by comparison with the microscopic agglutination assay (MAT), using a positive cut-off titer of ≥800. Banked serum samples from dogs exhibiting clinical signs and suspected leptospirosis were selected to form three groups based on MAT titer: (1) positive (n = 50); (2) borderline (n = 35); and (3) negative (n = 50). Using an analysis to weight group sizes to reflect French prevalence, the sensitivity and specificity were 98% and 93.5% (88.2% unweighted), respectively. This test rapidly identifies cases of acute canine leptospirosis with high levels of sensitivity and specificity with no interference from previous vaccination.
一种快速检测 IgM 的免疫层析试验（WITNESS® 钩端螺旋体检测试剂，硕腾公司）最近已可用于在护理点识别急性犬钩端螺旋体病。通过与显微镜凝集试验（MAT）比较，以≥800 的阳性截断滴度评估该试验的诊断敏感性和特异性。从表现出临床症状并疑似钩端螺旋体病的犬只中选取储存血清样本，根据 MAT 滴度分为三组：（1）阳性（n = 50）；（2）临界值（n = 35）；（3）阴性（n = 50）。采用加权组大小以反映法国患病率的分析方法，敏感性和特异性分别为 98%和 93.5%（未加权时为 88.2%）。该试验能快速识别急性犬钩端螺旋体病病例，具有高敏感性和特异性，且不受先前疫苗接种的干扰。
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