Zhang Li, Liang Shuang, Liu Ruiqing, Yuan Tianmeng, Zhang Shulai, Xu Zushun, Xu Haibo
Hubei Collaborative Innovation Center for Advance Organic Chemical Materials; Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan, Hubei 430062, China.
Department of Radiology at Union Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
Colloids Surf B Biointerfaces. 2016 Aug 1;144:344-354. doi: 10.1016/j.colsurfb.2016.04.014. Epub 2016 Apr 8.
Molecular imaging is of significant importance for early detection and diagnosis of cancer. Herein, a novel core-shell magnetic microsphere for dual modal magnetic resonance imaging (MRI) and optical imaging was produced by one-pot emulsifier-free emulsion polymerization, which could provide high resolution rate of histologic structure information and realize high sensitive detection at the same time. The synthesized magnetic microspheres composed of cores containing oleic acid (OA) and sodium undecylenate (NaUA) modified Fe3O4 nanoparticles and styrene (St), Glycidyl methacrylate (GMA), and polymerizable lanthanide complexes (Gd(AA)3Phen and Eu(AA)3Phen) polymerized on the surface for outer shells. Fluorescence spectra show characteristic emission peaks from Eu(3+) at 590nm and 615nm and vivid red fluorescence luminescence can be observed by 2-photon confocal scanning laser microscopy (CLSM). In vitro cytotoxicity tests based on the MTT assay demonstrate good cytocompatibility, the composites have longitudinal relaxivity value (r1) of 8.39mM(-1)s(-1) and also have transverse relaxivity value (r2) of 71.18mM(-1)s(-1) at clinical 3.0 T MR scanner. In vitro and in vivo MRI studies exhibit high signal enhancement on both T1- and T2-weighted MR images. These fascinating multifunctional properties suggest that the polymer microspheres have large clinical potential as multi-modal MRI/optical probes.
分子成像对于癌症的早期检测和诊断具有重要意义。在此，通过一锅法无乳化剂乳液聚合制备了一种用于双模态磁共振成像（MRI）和光学成像的新型核壳磁性微球，其能够提供高分辨率的组织结构信息并同时实现高灵敏度检测。合成的磁性微球由含有油酸（OA）和十一烯酸钠（NaUA）修饰的Fe3O4纳米粒子的核以及在表面聚合的苯乙烯（St）、甲基丙烯酸缩水甘油酯（GMA）和可聚合镧系配合物（Gd(AA)3Phen和Eu(AA)3Phen）组成的外壳。荧光光谱显示Eu(3+)在590nm和615nm处有特征发射峰，并且通过双光子共聚焦扫描激光显微镜（CLSM）可以观察到鲜艳的红色荧光发光。基于MTT法的体外细胞毒性测试表明其具有良好的细胞相容性，在临床3.0 T MR扫描仪上，该复合材料的纵向弛豫率值（r1）为8.39mM(-1)s(-1)，横向弛豫率值（r2）为71.18mM(-1)s(-1)。体外和体内MRI研究在T1加权和T2加权MR图像上均显示出高信号增强。这些引人入胜的多功能特性表明，聚合物微球作为多模态MRI/光学探针具有巨大的临床潜力。
