Zhao Juan, Liu Tao, Pan Lei, Jin Bai-hui, Zhao Dan, Chen Chen, Zhu You-yong, He Xia-hong
Ying Yong Sheng Tai Xue Bao. 2015 Dec;26(12):3737-45.
To reveal root endophytic and rhizosphere bacteria constitution of rice landraces in Yuanyang Terrace, isolation was carried out by tissue isolation method and soil dilution plate method for two landraces of Yuelianggu and Hongjiaolaojing. A total of 399 bacterial strains isolated were identified by morphological characteristics, physiological and biochemical identification. The results showed that there were 8 genera isolated from the root of Yuelianggu and 5 genera from its rhizosphere soil, and 5 genera were same. For Hongjiaolaojing, there were 10 genera isolated from its root and 7 genera from its rhizosphere soil, and 6 genera were same. By molecular biology, identification, a total of 11 species and 5 genera were isolated from the root of Yuelianggu, 8 species and 4 genera from its rhizosphere soil, and 5 species and 4 genera were same. As for Hongjiaolaojing, there were 9 species and 5 genera isolated from its root, and 10 species and 3 genera from its rhizosphere soil, and 4 species and 2 genera were same. The results of physiological and biochemical characteristics identification method and molecular identification method were basically same at the genus level, while most of the strains could be identified to species by molecular identification. There were certain species homology and specificity in the root endophytic and rhizosphere bacteria of Yuanyang rice landraces.
为揭示元阳梯田水稻地方品种的根内生细菌和根际细菌组成，采用组织分离法和土壤稀释平板法，对月亮谷和红脚老粳两个地方品种进行分离。通过形态特征、生理生化鉴定，对分离得到的399株细菌菌株进行了鉴定。结果表明，从月亮谷根部分离到8个属，根际土壤分离到5个属，其中有5个属相同；红脚老粳根部分离到10个属，根际土壤分离到7个属，其中有6个属相同。通过分子生物学鉴定，月亮谷根部共分离到11个种5个属，根际土壤分离到8个种4个属，其中有5个种4个属相同；红脚老粳根部分离到9个种5个属，根际土壤分离到10个种3个属，其中有4个种2个属相同。生理生化特征鉴定方法和分子鉴定方法的结果在属水平上基本一致，而分子鉴定能将大多数菌株鉴定到种。元阳水稻地方品种的根内生细菌和根际细菌存在一定的种属同源性和特异性。
