Yap Wing-Fai, Tay Vangene, Tan Sie-Hui, Yow Yoon-Yen, Chew Jactty
Department of Biological Sciences, Sunway University, Bandar Sunway, Selangor 47500, Malaysia.
Antibiotics (Basel). 2019 Sep 17;8(3):152. doi: 10.3390/antibiotics8030152.
Seaweeds are gaining a considerable amount of attention for their antioxidant and antibacterial properties. and , also known as 'sea grapes', are green seaweeds commonly found in different parts of the world, but the antioxidant and antibacterial potentials of Malaysian and have not been thoroughly explored. In this study, crude extracts of the seaweeds were prepared using chloroform, methanol, and water. Total phenolic content (TPC) and total flavonoid content (TFC) were measured, followed by in vitro antioxidant activity determination using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Antibacterial activities of these extracts were tested against Methicillin-resistant (MRSA) and neuropathogenic K1. Liquid chromatography-mass spectrometry (LCMS) analysis was then used to determine the possible compounds present in the extract with the most potent antioxidant and antibacterial activity. Results showed that chloroform extract had the highest TPC (13.41 ± 0.86 mg GAE/g), antioxidant effect (EC at 0.65 ± 0.03 mg/mL), and the strongest antibacterial effect (97.7 ± 0.30%) against MRSA. LCMS analysis proposed that the chloroform extracts of are mainly polyunsaturated and monounsaturated fatty acids, terpenes, and alkaloids. In conclusion, can be a great source of novel antioxidant and antibacterial agents, but isolation and purification of the bioactive compounds are needed to study their mechanism of action.
海藻因其抗氧化和抗菌特性而受到广泛关注。 ，也被称为“海葡萄”，是一种常见于世界各地的绿色海藻，但马来西亚的 和 的抗氧化和抗菌潜力尚未得到充分研究。在本研究中，使用氯仿、甲醇和水制备了海藻的粗提物。测定了总酚含量（TPC）和总黄酮含量（TFC），随后使用2,2-二苯基-1-苦基肼基（DPPH）自由基清除法测定体外抗氧化活性。测试了这些提取物对耐甲氧西林金黄色葡萄球菌（MRSA）和神经致病性大肠杆菌K1的抗菌活性。然后使用液相色谱-质谱（LCMS）分析来确定提取物中具有最有效抗氧化和抗菌活性的可能化合物。结果表明， 氯仿提取物具有最高的TPC（13.41±0.86 mg GAE/g）、抗氧化效果（EC50为0.65±0.03 mg/mL），并且对MRSA的抗菌效果最强（97.7±0.30%）。LCMS分析表明， 的氯仿提取物主要是多不饱和和单不饱和脂肪酸、萜类化合物和生物碱。总之， 可以成为新型抗氧化剂和抗菌剂的重要来源，但需要对生物活性化合物进行分离和纯化以研究其作用机制。
