Department of Food Science and Nutrition, Institute of Food Technology of Plant Origin, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, Poland.
Carbohydr Polym. 2016 Aug 1;146:310-9. doi: 10.1016/j.carbpol.2016.03.015. Epub 2016 Mar 9.
Some ß-glucans can be easily extracted from Basidiomycete mushrooms but commonly used extraction procedures are not satisfactory. A simultaneous method for acid extraction and deproteinization in the case of Pleurotus ostreatus was developed using response surface methodology. The optimized extraction conditions proposed here (30°C, 3.8% HCl, 300min, stirring) allow for the simultaneous extraction and deproteinization of polysaccharides. Additionally, the acid extraction yield was 7 times greater than that of hot water extraction. The combined enzymatic digestion with lyticase, ß-glucanase, exo-1,3-ß-d-glucanase, and ß-glucosidase results elucidated that an extract containing ß-1,3-ß-1,6-ß-1,4-glucan. The gel permeation chromatography (GPC) results showed that the two glucan fractions obtained do not contain linked proteins. The weight average molecular weight of the first fraction (Mw=1137kDa) was 60 times higher than that of the second fraction (Mw=19kDa).
一些β-葡聚糖可以很容易地从担子菌蘑菇中提取出来，但常用的提取方法并不令人满意。本研究采用响应面法开发了一种同时进行酸提取和蛋白去除的方法。这里提出的优化提取条件（30°C、3.8%HCl、300min、搅拌）可以实现多糖的同时提取和去蛋白。此外，酸提取的产率是热水提取的 7 倍。用溶菌酶、β-葡聚糖酶、外切 1,3-β-葡聚糖酶和β-葡萄糖苷酶联合酶解的结果表明，提取物中含有β-1,3-β-1,6-β-1,4-葡聚糖。凝胶渗透色谱（GPC）结果表明，得到的两种葡聚糖级分不含连接蛋白。第一级分（Mw=1137kDa）的重均分子量比第二级分（Mw=19kDa）高 60 倍。
