Liu Yanfang, Tang Qingjiu, Yang Yan, Zhou Shuai, Wu Di, Tang Chuanhong, Zhang Zhong, Yan Mengqiu, Feng Jie, Zhang Jing-Song
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungi Processing, Shanghai, China; School of Chemical and Material Engineering, State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, China.
Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Key Laboratory of Edible Fungi Resources and Utilization (South), Ministry of Agriculture, National Engineering Research Center of Edible Fungi, National R&D Center for Edible Fungi Processing, Shanghai, China.
Int J Med Mushrooms. 2017;19(1):75-85. doi: 10.1615/IntJMedMushrooms.v19.i1.80.
Molecular weight (Mw) distributions of polysaccharides from the fruiting bodies of different Ganoderma lucidum strains and G. sinense were investigated and compared using high-pressure size exclusion chromatography/multiangle laser light scattering/refractive index analysis. Results showed that there were big differences in the Mw distributions and characteristics of polysaccharides from 2 species of Ganoderma. All tested G. lucidum materials exhibited similar polysaccharide distributions and similar characteristics for each fraction. The fraction with highest Mw (peak 1) was identified as β-(1→3)-linked D-glucan with (1→6)-β-D-glucopyranosyl side branches. G. sinense fruiting bodies did not include the β-D-glucan when compared with G. lucidum. A high-pressure size exclusion chromatography method was developed and applied to determine the amount of high-Mw β-D-glucan in G. lucidum fruiting bodies. Results indicated that there was no obvious relationship between β-D-glucan content and the genetic similarity of G. lucidum. The strain labeled "Longzhi no. 2" was determined to possess the largest amount of β-D-glucan: 8.2 mg/mL based on the dry weight of fruiting bodies. The β-D-glucan content in the hot water extract of Longzhi no. 2 reached 17.05%. For the "Hunong no. 1" strain, the β-D-glucan content in log-cultivated fruiting bodies was much higher than that in bag-cultivated ones. This method could be used to improve quality control of polysaccharides in G. lucidum.
采用高压尺寸排阻色谱/多角度激光光散射/示差折光检测联用技术，对不同灵芝菌株及紫芝子实体多糖的分子量（Mw）分布进行了研究和比较。结果表明，两种灵芝多糖的Mw分布和特性存在较大差异。所有测试的灵芝材料的多糖分布和各组分特性相似。Mw最高的组分（峰1）被鉴定为具有（1→6）-β-D-吡喃葡萄糖基侧链的β-（1→3）连接的D-葡聚糖。与灵芝相比，紫芝子实体不含β-D-葡聚糖。建立了一种高压尺寸排阻色谱法，用于测定灵芝子实体中高Mwβ-D-葡聚糖的含量。结果表明，β-D-葡聚糖含量与灵芝的遗传相似性之间没有明显关系。标记为“龙芝2号”的菌株被测定含有最大量的β-D-葡聚糖：基于子实体干重为8.2 mg/mL。龙芝2号热水提取物中的β-D-葡聚糖含量达到17.05%。对于“沪农1号”菌株，原木栽培子实体中的β-D-葡聚糖含量远高于袋栽子实体。该方法可用于改善灵芝多糖的质量控制。
