Erdogan Sevil, Kaya Murat
Fisheries Programme, Keşan Vocational College, Trakya University, 22800 Keşan, Edirne, Turkey.
Department of Biotechnology and Molecular Biology, Faculty of Science and Letters, Aksaray University, 68100 Aksaray, Turkey.
Int J Biol Macromol. 2016 Aug;89:118-26. doi: 10.1016/j.ijbiomac.2016.04.059. Epub 2016 Apr 22.
This is the first study to explain the differences in the physicochemical properties of chitin and chitosan obtained from the nymphs and adults of Dociostaurus maroccanus using the same method. Fourier transform infrared spectroscopy, thermogravimetric analysis and x-ray diffraction analysis results demonstrated that the chitins from both the adults and nymphs were in the α-form. The chitin contents of the adults (14%) and nymphs (12%) were of the same order of magnitude. The crystalline index values of chitins from the adult and nymph grasshoppers were 71% and 74%, respectively. Thermal stabilities of the chitins and chitosans from adult and nymph grasshoppers were close to each other. Both the adult (7.2kDa) and nymph (5.6kDa) chitosans had low molar masses. Environmental scanning electron microscopy revealed that the surface morphologies of both chitins consisted of nanofibers and nanopores together, and they were very similar to each other. Consequently, it was determined that the physicochemical properties of the chitins and chitosans from adults and nymphs of D. maroccanus were not very different, so it can be hypothesized that the development of the chitin structure in the nymph has almost been completed and the nymph chitin has the same characteristics as the adult.
这是第一项使用相同方法解释从摩洛哥戟纹蝗若虫和成虫中获得的几丁质和壳聚糖理化性质差异的研究。傅里叶变换红外光谱、热重分析和X射线衍射分析结果表明，成虫和若虫的几丁质均为α型。成虫（14%）和若虫（12%）的几丁质含量处于同一数量级。成虫和若虫蝗虫的几丁质结晶指数值分别为71%和74%。成虫和若虫蝗虫的几丁质和壳聚糖的热稳定性彼此接近。成虫（7.2 kDa）和若虫（5.6 kDa）的壳聚糖摩尔质量均较低。环境扫描电子显微镜显示，两种几丁质的表面形态均由纳米纤维和纳米孔共同组成，且彼此非常相似。因此，确定摩洛哥戟纹蝗成虫和若虫的几丁质和壳聚糖的理化性质差异不大，所以可以推测若虫中几丁质结构的发育几乎已经完成，且若虫几丁质具有与成虫相同的特征。
