Interdisciplinary Research Laboratory in Bioresources Environment and Materials (LIRBEM), ENS, Cadi Ayyad University, Hay Hassani, Route d'Essaouira, Marrakech 40000, Morocco; Bioresource and Food Safety Laboratory, Cadi Ayyad University, Faculty of Sciences and Technologies, 112 Boulevard Abdelkrim Al Khattabi, 40000 Marrakech, Morocco.
University of Pau and Adour Countries (UPPA), IPREM, Hélioparc Pau Pyrénées, Pau, France.
Int J Biol Macromol. 2024 May;266(Pt 2):131314. doi: 10.1016/j.ijbiomac.2024.131314. Epub 2024 Apr 2.
Breeding of the black soldier fly is carried out to produce proteins. It is accompanied by releases during the life cycle of this insect. This work is a study of the valorization of these rejects through the production of chitins and chitosans with controlled characteristics. An extraction process is developed with an order of treatments and reaction conditions that provide chitins with high contents. These contents increase as the stages of the life cycle progress and drop for the adult. However, the exuviae chitins present organic impurities which will be eliminated at the N-deacetylation reaction for pupe and after a purification treatment for chitosan from larval stages. All these chitins have an α structure although certain physicochemical characteristics of the larval exuviae chitins are close to those presented by γ chitin. The observed shifts are linked to the effect of impurities rather than to a difference in structure. N-deacetylation of chitins makes possible the valorization of all rejects by the production of pure chitosans with high yields which retain a porous structure for the exuviae and fibrous for the adult which allow complementary applications. These chitosans are highly to completely deacetylated and their molar masses can vary depending on the process and life stage.
黑蝇的繁殖是为了生产蛋白质。在这种昆虫的生命周期中伴随着释放。这项工作是通过生产具有控制特性的壳聚糖和壳聚糖来利用这些废物。开发了一种提取工艺，采用一系列处理和反应条件，提供高含量的壳聚糖。这些含量随着生命周期阶段的进展而增加，对于成虫则下降。然而，蜕皮壳聚糖含有有机杂质，这些杂质将在蛹的 N-去乙酰化反应和幼虫阶段的壳聚糖纯化处理中被去除。所有这些壳聚糖都具有α结构，尽管幼虫蜕皮壳聚糖的某些物理化学特性与γ壳聚糖接近。观察到的转变与杂质的影响有关，而不是结构上的差异。壳聚糖的 N-去乙酰化使通过生产具有高收率的纯壳聚糖来利用所有废物成为可能，这些壳聚糖保留了蜕皮的多孔结构和成虫的纤维状结构，允许互补的应用。这些壳聚糖高度至完全去乙酰化，其摩尔质量可以根据工艺和生命阶段而变化。
