Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran.
College of Science, University of Tehran, Tehran, Iran.
Appl Biochem Biotechnol. 2023 May;195(5):3047-3066. doi: 10.1007/s12010-022-04246-3. Epub 2022 Dec 12.
In this study, the chitin of adult Mediterranean flour moth (Ephestia kuheniella) (Cht) was extracted and then converted to chitosan by deacetylation process to achieve the chitosan derived from E. kuheniella (Chs). The new chitosan-based scaffold was produced using the polyvinyl alcohol (PVA) co-electrospinning technique. The degree of deacetylation was obtained using the distillation-titration and Fourier transform infrared spectroscopy. The surface morphology and crystallinity index of Chs were observed using scanning electron microscopy and X-ray diffraction analysis, respectively, and compared with the commercial chitosan (Chs). Thermogravimetric analysis was used to estimate two chitosans' water content and thermal stability. The average molecular mass analysis was performed using viscometry. Moreover, the minimum inhibitory concentration and DPPH assay were used to study the antimicrobial activity and antioxidant potential of the Chs, respectively. Accordingly, Chs was smoother with fewer pores and flakes than Chs and its crystallinity index was higher than Chs. The water content and thermal stability were lower and similar for Chs compared to Chs. The average molecular mass of Chs was ~ 5.8 kDa, making it classified as low molecular weight chitosan. The antimicrobial activity of Chs against a representative Gram-negative bacteria; E. coli resulted to be the same as Chs. However, less effective than Chs against a representative Gram-positive bacteria is S. aureus. The Chs/PVA ratio scaffold was optimized at 30:70 to fabricate a uniform nanofiber scaffold.
在这项研究中，从成年地中海粉斑螟（Ephestia kuheniella）（Cht）中提取甲壳素，然后通过脱乙酰化过程将其转化为壳聚糖，从而获得源自 E. kuheniella 的壳聚糖（Chs）。使用聚乙烯醇（PVA）共电纺技术生产新的壳聚糖基支架。通过蒸馏滴定和傅里叶变换红外光谱法获得脱乙酰度。使用扫描电子显微镜观察 Chs 的表面形态和结晶度指数，并用 X 射线衍射分析进行比较与商业壳聚糖（Chs）。使用热重分析估计两种壳聚糖的含水量和热稳定性。使用粘度法进行平均分子量分析。此外，使用最低抑菌浓度和 DPPH 测定法分别研究 Chs 的抗菌活性和抗氧化潜力。因此，Chs 比 Chs 更光滑，孔隙和薄片更少，其结晶度指数高于 Chs。Chs 的含水量和热稳定性均低于 Chs，但与 Chs 相似。Chs 的平均分子量约为 5.8 kDa，属于低分子量壳聚糖。Chs 对代表性革兰氏阴性细菌 E. coli 的抗菌活性与 Chs 相同。然而，对代表性革兰氏阳性菌 S. aureus 的效果不如 Chs。Chs/PVA 比例支架优化为 30:70 以制造均匀的纳米纤维支架。
