Song Ningning, Liang Bing, Wang Dandan
Department of Breast Surgery, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China.
Pak J Pharm Sci. 2016 Mar;29(2 Suppl):679-84.
This paper is to detect the expression differences of serum miR-21 in breast cancer, in order to further clarify the function of miR-21 in familial and TNBC pathogenesis of breast cancer. The serum had been collected for healthy check-up females, women with high risk of breast cancer and different types of breast cancer patients. Nematodes were taken as the external reference, real-time fluorescent quantitative PCR detection were taken for expression level of miR-21 in 77 cases of serum. The miR-21 expression level of familial breast cancer group, TNBC group and breast cancer high risk group were significantly higher than that in normal control group and other breast cancer group, P<0.01. Serum miR-21 expression level was associated with lymph node metastasis and Ki67 high expression, P<0.01. Results had proved that serum miR-21 expression quantity increased in familial breast cancer and TNBC and was correlated with lymph node metastasis and Ki67 expression. Serum miR-21 was closed related with TNBC and familial breast cancer. The relative expression quantity of miR-21 in breast cancer serum had no obvious relation with unilateral or bilateral tumor and menstrual situation. Its increased expression might be correlated to the breast cancer hereditary, malignant degree and prognosis judgement and its mechanism required further research.
本文旨在检测血清miR-21在乳腺癌中的表达差异，以进一步阐明miR-21在乳腺癌家族性及三阴性乳腺癌（TNBC）发病机制中的作用。收集了健康体检女性、乳腺癌高危女性及不同类型乳腺癌患者的血清。以线虫为外参，采用实时荧光定量PCR检测77例血清中miR-21的表达水平。家族性乳腺癌组、TNBC组及乳腺癌高危组的miR-21表达水平显著高于正常对照组及其他乳腺癌组，P<0.01。血清miR-21表达水平与淋巴结转移及Ki67高表达相关，P<0.01。结果表明，家族性乳腺癌和TNBC中血清miR-21表达量增加，且与淋巴结转移及Ki67表达相关。血清miR-21与TNBC及家族性乳腺癌密切相关。乳腺癌血清中miR-21的相对表达量与肿瘤单侧或双侧及月经情况无明显关系。其表达增加可能与乳腺癌遗传、恶性程度及预后判断相关，其机制有待进一步研究。
