Wang Yujie, Zhang Bowen, Zhang Xiaoping, Wang Xuemei, Cheng Jian, Chen Baoan
Department of hematology and oncology (key Discipline of Jiangshu Province), Zhongda Hospital, School of Medicine, Southeast University, Nangjing, Jiangsu Province, People's Republic of China.
State Key Laboratory of Bioelectronics (Chien-Shiung Wu Laboratory), Southeast University, Nanjing, Jiangsu Province, People's Republic of China.
PLoS One. 2016 Apr 26;11(4):e0153821. doi: 10.1371/journal.pone.0153821. eCollection 2016.
Develop and evaluate an electrochemical method to identify healthy individuals, malignant hematopathic patients and solid tumor patients by detecting the leukocytes in whole-blood.
A total of 114 individual blood samples obtained from our affiliated hospital in China (June 2015- August 2015) were divided into three groups: healthy individuals (n = 35), hematologic malignancies (n = 41) and solid tumors (n = 38). An electrochemical workstation system was used to measure differential pulse voltammetry due to the different electrochemical behaviors of leukocytes in blood samples. Then, one-way analysis of variance (ANOVA) was applied to analyze the scanning curves and to compare the peak potential and peak current.
The scanning curve demonstrated the specific electrochemical behaviors of the blank potassium ferricyanide solution and that mixed with blood samples in different groups. Significant differences in mean peak potentials of mixture and shifts (ΔEp (mV)) were observed of the three groups (P< = 0.001). 106.00±9.00 and 3.14±7.48 for Group healthy individuals, 120.90±11.18 and 18.10±8.81 for Group hematologic malignancies, 136.84±11.53 and 32.89±10.50 for Group solid tumors, respectively. In contrast, there were no significant differences in the peak currents and shifts.
The newly developed method to apply the electrochemical workstation system to identify hematologic malignancies and solid tumors with good sensitivity and specificity might be effective, suggesting a potential utility in clinical application.
开发并评估一种通过检测全血中的白细胞来鉴别健康个体、恶性血液病患者和实体肿瘤患者的电化学方法。
从我国附属医院（2015年6月 - 2015年8月）获取的114份个体血样被分为三组：健康个体（n = 35）、血液系统恶性肿瘤患者（n = 41）和实体肿瘤患者（n = 38）。由于血样中白细胞具有不同的电化学行为，使用电化学工作站系统测量差分脉冲伏安法。然后，应用单因素方差分析（ANOVA）来分析扫描曲线并比较峰电位和峰电流。
扫描曲线显示了空白铁氰化钾溶液以及与不同组血样混合后的溶液的特定电化学行为。观察到三组混合物的平均峰电位和偏移量（ΔEp（mV））存在显著差异（P <= 0.001）。健康个体组分别为106.00±9.00和3.14±7.48，血液系统恶性肿瘤组分别为120.90±11.18和18.10±8.81，实体肿瘤组分别为136.84±11.53和32.89±10.50。相比之下，峰电流和偏移量没有显著差异。
新开发的应用电化学工作站系统鉴别血液系统恶性肿瘤和实体肿瘤的方法具有良好的灵敏度和特异性，可能是有效的，提示其在临床应用中具有潜在用途。
