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不同食用蘑菇品种中谷胱甘肽、硒和丙二醛的测定

Determination of Glutathione, Selenium, and Malondialdehyde in Different Edible Mushroom Species.

作者信息

Dogan Hacer, Coteli Ebru, Karatas Fikret

机构信息

Faculty of Science, Department of Chemistry, Firat University, 23119,, Elazıg, Turkey.

出版信息

Biol Trace Elem Res. 2016 Dec;174(2):459-463. doi: 10.1007/s12011-016-0715-2. Epub 2016 Apr 26.

Abstract

In this study, the amount of reduced glutathione (GSH), oxidized glutathione (GSSG), and malondialdehyde (MDA) were determined by high performance liquid chromatography (HPLC), and selenium was determined by using the fluorescence spectrophotometer in eight different species of edible mushrooms. Brittlegill mushroom (Russula delica), meadow mushroom (Agaricus campestris), dryad's saddle mushroom (Polyporus squamosus), white button mushroom (Agaricus bisporus), Pleurotus spp., ink mushroom (Coprinus atramentarius), ebekari mushroom (slimy) (Elazığ local) and çaşır mushroom (Pleurotus eryngii) (Tunceli local) were used for analysis. The amounts of GSH, GSSG, Se, and MDA with GSH/GSSG ratio in the eight different species of edible mushrooms were observed in between 269.10 ± 16.94-1554.83 ± 58.12 μg/g; 23.55 ± 1.89-841.90 ± 20.03 μg/g; 15.06 ± 1.56-82.10 ± 3.84 μg/g; 5.46 ± 0.50-27.45 ± 2.58 μg/g wet weight and 0.32-41.35, respectively. There is a weak correlation (R  = 0.389) between MDA and Se, on the other hand, the correlation (R  = 0.831) between GSH/GSSG ratio and selenium in mushrooms are reasonable well. In a similar manner, there is a weak correlation (R  = 0551) between GSH/GSSG and MDA ratios in mushrooms. It was found that these edible mushroom species are good source of glutathione (GSH, GSSG), and selenium (Se) in terms of quantities obtained; therefore, it can be said that mushrooms are a rich source of antioxidants.

摘要

在本研究中,采用高效液相色谱法(HPLC)测定了八种不同食用蘑菇中还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)和丙二醛(MDA)的含量,并使用荧光分光光度计测定了硒含量。分析使用了脆褶菇(Russula delica)、草地蘑菇(Agaricus campestris)、干巴菌(Polyporus squamosus)、白蘑菇(Agaricus bisporus)、侧耳属蘑菇、墨汁鬼伞(Coprinus atramentarius)、埃贝卡里蘑菇(黏滑的)(埃拉泽当地品种)和察希尔蘑菇(杏鲍菇)(通杰利当地品种)。观察到八种不同食用蘑菇中GSH、GSSG、硒和MDA的含量以及GSH/GSSG比值分别在湿重269.10±16.94 - 1554.83±58.12μg/g;23.55±1.89 - 841.90±20.03μg/g;15.06±1.56 - 82.10±3.84μg/g;5.46±0.50 - 27.45±2.58μg/g和0.32 - 41.35之间。MDA与硒之间存在弱相关性(R = 0.389),另一方面,蘑菇中GSH/GSSG比值与硒之间的相关性(R = 0.831)相当良好。同样,蘑菇中GSH/GSSG与MDA比值之间存在弱相关性(R = 0.551)。从所获得的量来看,发现这些食用蘑菇品种是谷胱甘肽(GSH、GSSG)和硒(Se)的良好来源;因此,可以说蘑菇是丰富的抗氧化剂来源。

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