Lu Fang, Sun Xin, Dai Xiaodong, Zhang Piqi, Ma Yinpeng, Xu Yafei, Wang Lei, Zhang Jiechi
Institute of Microbiology, Heilongjiang Academy of Sciences, Harbin 150010, China.
Department of Biotechnology, Institute of Advanced Technology, Heilongjiang Academy of Sciences, Harbin 150001, China.
J Fungi (Basel). 2025 Feb 20;11(3):167. doi: 10.3390/jof11030167.
High-temperature stress is a key factor that reduces the yields of edible fungi. () is a nutrient-rich edible fungus that is widely cultivated in China. In this study, we analyzed the physiological, transcriptomic, and metabolomic results of (variety "Hei29") under high-temperature stress. Our findings revealed that high temperatures (30 °C and 35 °C) significantly reduced hyphal growth, increased malondialdehyde content and antioxidant enzyme activity, and enhanced the accumulation of secondary metabolites, such as phenolic compounds and flavonoids. A total of 15 candidate genes potentially responsive to high-temperature stress were identified through transcriptomic analysis, including those involved in regulating antioxidant defense, heat shock response, sugar metabolism, amino acid metabolism, and accumulating secondary metabolites. Metabolomic analysis identified three candidate metabolites potentially responsive to high-temperature stress, including kinetin, flavonoids, and caffeic acid, as well as several metabolic pathways, including nucleotide metabolism, ABC transporters, and cofactor biosynthesis. These mechanisms help mitigate oxidative damage to cellular structures and energy deficits caused by elevated temperatures, enabling the fungus to maintain cellular stability, metabolic function, and growth under heat stress. This study is the first to explore the molecular mechanism of in response to high-temperature stress. The results provide valuable insights into the molecular mechanisms of heat stress tolerance in , highlighting potential targets for developing heat-tolerant strains for industrial application.
高温胁迫是降低食用菌产量的关键因素。（）是一种营养丰富的食用菌，在中国广泛种植。在本研究中，我们分析了（品种“Hei29”）在高温胁迫下的生理、转录组和代谢组结果。我们的研究结果表明，高温（30℃和35℃）显著降低了菌丝生长，增加了丙二醛含量和抗氧化酶活性，并促进了次生代谢产物如酚类化合物和黄酮类化合物的积累。通过转录组分析共鉴定出15个可能对高温胁迫有响应的候选基因，包括参与调节抗氧化防御、热休克反应、糖代谢、氨基酸代谢以及积累次生代谢产物的基因。代谢组分析鉴定出三种可能对高温胁迫有响应的候选代谢物，包括激动素、黄酮类化合物和咖啡酸，以及几条代谢途径，包括核苷酸代谢、ABC转运蛋白和辅因子生物合成。这些机制有助于减轻高温对细胞结构造成的氧化损伤和能量不足，使真菌能够在热胁迫下维持细胞稳定性、代谢功能和生长。本研究首次探索了（）对高温胁迫的分子机制。研究结果为（）耐热性的分子机制提供了有价值的见解，突出了开发工业应用耐热菌株的潜在靶点。
