Phenylobacterium panacis sp. nov., isolated from the rhizosphere of rusty mountain ginseng.

A novel, Gram-stain-negative, rod-shaped bacterial strain, designated as DCY109, was isolated from the rhizosphere of rusty mountain ginseng root located on Hwacheon mountain of Gangwon province, South Korea. 16S rRNA gene sequence analysis revealed that strain DCY109 belonged to the genus Phenylobacterium and was related closely to Phenylobacterium muchangponense KACC 15042 (98.2 % similarity), Phenylobacterium immobile DSM 1986 (96.9 %) and Phenylobacterium koreense KCTC 12206 (96.7 %). The predominant isoprenoid quinone was ubiquinone (Q-10) and the DNA G+C content was 66.9 mol%. The major polar lipids were phosphatidylglycerol, an unidentified glycolipid and an unidentified lipid. The major fatty acids (>10 %) were C16 : 0, summed feature 3 (which comprised C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 8 (which comprised C18 : 1ω7c and/or C18 : 1ω6c). Mean DNA-DNA relatedness between strain DCY109 and its closest relative, P. muchangponense KACC 15042, was 15.1±3.9 %. Based on the physiological, biochemical, chemotaxonomic and genetic analyses, strain DCY109 is considered to represent a novel species of the genus Phenylobacterium, for which the name Phenylobacterium panacis sp. nov. is proposed. The type strain is DCY109 (=KCTC 42749=JCM 31045).