Grinna L S, Tschopp J F
Salk Institute Biotechnology/Industrial Associates, Inc., La Jolla, California 92037.
Yeast. 1989 Mar-Apr;5(2):107-15. doi: 10.1002/yea.320050206.
The secreted glycoproteins of Pichia pastoris contain more than 35% of their N-linked oligosaccharides as structures smaller than Man14GlcNAc2 (Man = mannose; GlcNAc = N-acetylglucosamine). On heterologous invertase produced in P. pastoris, approximately 85% of the oligosaccharides are in the size range Man8-14GlcNAc2. The structures appear to contain alpha-linked mannose. In addition, one-third of the structures contain net negative charge and can be radio-labelled in vivo with 32P. The largest oligosaccharides isolated from P. pastoris are significantly shorter than the hypermannosylated structures typical of S. cerevisiae, indicating that the factors which influence the processing of N-linked oligosaccharides in P. pastoris are different from those which influence processing in S. cerevisiae. The smaller N-linked oligosaccharides synthesized by P. pastoris resemble high-mannose oligosaccharides synthesized by animal cells, and this finding increases the utility of P. pastoris as a host for the production of heterologous glycoproteins.
巴斯德毕赤酵母分泌的糖蛋白含有超过35%的N-连接寡糖，其结构小于Man14GlcNAc2（Man = 甘露糖；GlcNAc = N-乙酰葡糖胺）。在巴斯德毕赤酵母中产生的异源转化酶上，约85%的寡糖大小在Man8-14GlcNAc2范围内。这些结构似乎含有α-连接的甘露糖。此外，三分之一的结构带有净负电荷，并且在体内能用32P进行放射性标记。从巴斯德毕赤酵母中分离出的最大寡糖明显短于酿酒酵母典型的高甘露糖基化结构，这表明影响巴斯德毕赤酵母中N-连接寡糖加工的因素与影响酿酒酵母中加工的因素不同。巴斯德毕赤酵母合成的较小N-连接寡糖类似于动物细胞合成的高甘露糖寡糖，这一发现增加了巴斯德毕赤酵母作为生产异源糖蛋白宿主的实用性。
