Dehghan Firouzeh, Hajiaghaalipour Fatemeh, Yusof Ashril, Muniandy Sekaran, Hosseini Seyed Ali, Heydari Sedigheh, Salim Landa Zeenelabdin Ali, Azarbayjani Mohammad Ali
Department of Exercise Science, Sports Centre, University of Malaya, 50603 Kuala lumpur, Malaysia.
Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Sci Rep. 2016 Apr 28;6:25139. doi: 10.1038/srep25139.
Saffron is consumed as food and medicine to treat several illnesses. This study elucidates the saffron effectiveness on diabetic parameters in-vitro and combined with resistance exercise in-vivo. The antioxidant properties of saffron was examined. Insulin secretion and glucose uptake were examined by cultured RIN-5F and L6 myotubes cells. The expressions of GLUT2, GLUT4, and AMPKα were determined by Western blot. Diabetic and non-diabetic male rats were divided into: control, training, extract treatment, training + extract treatment and metformin. The exercise and 40 mg/kg/day saffron treatments were carried out for six weeks. The antioxidant capacity of saffron was higher compare to positive control (P < 0.01). High dose of saffron stimulated insulin release in RIN-5F cells and improved glucose uptake in L6 myotubes. GLUT4 and AMPKα expressions increased in both doses of saffron (P < 0.01), whereas GLUT2 not changed (p > 0.05). Serum glucose, cholesterol, triglyceride, low-density lipoprotein, very low-density lipoprotein, insulin resistance, and glycated hemoglobin levels decreased in treated rats compared to untreated (p < 0.01). However, no significant differences were observed in the high-density lipoprotein, insulin, adiponectin, and leptin concentration levels in all groups (p > 0.05). The findings suggest that saffron consuming alongside exercise could improve diabetic parameters through redox-mediated mechanisms and GLUT4/AMPK pathway to entrap glucose uptake.
藏红花作为食物和药物用于治疗多种疾病。本研究阐明了藏红花在体外对糖尿病参数的有效性以及在体内与抗阻运动联合使用时的效果。研究了藏红花的抗氧化特性。通过培养的RIN-5F和L6肌管细胞检测胰岛素分泌和葡萄糖摄取。通过蛋白质免疫印迹法测定GLUT2、GLUT4和AMPKα的表达。将糖尿病和非糖尿病雄性大鼠分为：对照组、训练组、提取物治疗组、训练 + 提取物治疗组和二甲双胍组。运动和每天40 mg/kg的藏红花治疗持续六周。与阳性对照相比，藏红花的抗氧化能力更高（P < 0.01）。高剂量藏红花刺激RIN-5F细胞中的胰岛素释放，并改善L6肌管中的葡萄糖摄取。两种剂量的藏红花均使GLUT4和AMPKα表达增加（P < 0.01），而GLUT2未发生变化（p > 0.05）。与未治疗的大鼠相比，治疗组大鼠的血清葡萄糖、胆固醇、甘油三酯、低密度脂蛋白、极低密度脂蛋白、胰岛素抵抗和糖化血红蛋白水平降低（p < 0.01）。然而，所有组的高密度脂蛋白、胰岛素、脂联素和瘦素浓度水平均未观察到显著差异（p > 0.05）。研究结果表明，与运动同时食用藏红花可通过氧化还原介导的机制和GLUT4/AMPK途径改善糖尿病参数，从而促进葡萄糖摄取。
