Mosher R A, Dewey D, Thormann W, Saville D A, Bier M
Anal Chem. 1989 Feb 15;61(4):362-6. doi: 10.1021/ac00179a015.
A mathematical model of the electrophoretic behavior of proteins is presented. The Debye-Hückel-Henry theory is used for the description of protein mobility, which has the important result of making net mobility a function of ionic strength. A net charge vs pH relationship and a diffusion coefficient are required to describe a specific protein. The model is employed for the computer simulation of three distinct electrophoretic modes: isoelectric focusing, isotachophoresis, and zone electrophoresis. The validity of the model is tested by comparing simulation with experimental data. Excellent qualitative agreement was found.
本文提出了一种蛋白质电泳行为的数学模型。采用德拜 - 休克尔 - 亨利理论来描述蛋白质迁移率，该理论的一个重要结果是使净迁移率成为离子强度的函数。描述特定蛋白质需要净电荷与pH值的关系以及扩散系数。该模型用于三种不同电泳模式的计算机模拟：等电聚焦、等速电泳和区带电泳。通过将模拟结果与实验数据进行比较来检验模型的有效性。结果发现两者在定性上吻合得很好。
