Tang X M, Clermont Y
Department of Anatomy, McGill University, Montreal, Quebec, Canada.
Anat Rec. 1989 Feb;223(2):128-38. doi: 10.1002/ar.1092230204.
The formation of granules in neutrophil (heterophil) progenitor cells was examined with the electron microscope in sections of rat bone marrow fixed in 2% glutaraldehyde and postfixed with reduced osmium (Karnovsky: Proceedings of the 11th Meeting, American Society of Cell Biologists, Abstr. 284, p. 146, 1971). The cells were also osmicated in 2% osmium tetroxide for 36 hours at 37 degrees C to outline the osmiophilic element usually observed on the cis-face of the stacks of saccules of the Golgi apparatus of various cell types. In myeloblasts, which do not produce granules, the cis-osmiophilic element (CE) was found on the concave face of the C-shaped Golgi stacks. In promyelocytes the CE was present on the convex aspect of the C-shaped stacks, while the primary (azurophilic) granules formed in relation to elements on the concave aspects of the stacks. In myelocytes, the situation was reversed: the CE was found on the concave face of the Golgi stacks, while the secondary (specific) granules were seen forming in relation to elements on the convex aspect of the stacks. Finally, in metamyelocytes and mature neutrophils in which no granule formation took place, the appearance on Golgi stacks varied: they were either flat or C-shaped. The CE was indiscriminately found on one face or the other of the flat Golgi stacks of metamyetocytes and on the convex or concave faces of the C-shaped Golgi stacks of mature neutrophils. Using the cis-osmiophilic-element as a marker of the cis-face of the stacked Golgi elements, it thus appeared that despite marked changes in the configuration and orientation of the stacks of the cis-trans polarity of the stacked elements was maintained throughout granulopoiesis. In addition the primary and secondary granules that appeared sequentially in promyelocytes and myelocytes were both seen to form in relation to trans-elements of the Golgi apparatus.
采用电子显微镜，在经2%戊二醛固定、用还原锇后固定（卡诺夫斯基：《第11届会议论文集》，美国细胞生物学家协会，摘要284，第146页，1971年）的大鼠骨髓切片中，检查嗜中性（异嗜性）祖细胞中颗粒的形成情况。细胞还在2%四氧化锇中于37℃处理36小时，以勾勒出通常在各种细胞类型高尔基体囊泡堆叠顺面观察到的嗜锇性成分。在不产生颗粒的原粒细胞中，顺嗜锇性成分（CE）位于C形高尔基体堆叠的凹面上。在早幼粒细胞中，CE存在于C形堆叠的凸面上，而初级（嗜天青）颗粒则相对于堆叠凹面上的成分形成。在中幼粒细胞中，情况相反：CE位于高尔基体堆叠的凹面上，而次级（特异性）颗粒则相对于堆叠凸面上的成分形成。最后，在不发生颗粒形成的晚幼粒细胞和成熟嗜中性粒细胞中，高尔基体堆叠的外观各不相同：它们要么是扁平的，要么是C形的。在晚幼粒细胞扁平的高尔基体堆叠的这一面或那一面以及成熟嗜中性粒细胞C形高尔基体堆叠的凸面或凹面上均可随意发现CE。因此，以顺嗜锇性成分作为堆叠高尔基体成分顺面的标记，似乎尽管堆叠的构型和方向发生了显著变化，但堆叠成分的顺 - 反极性在整个粒细胞生成过程中得以维持。此外，在早幼粒细胞和中幼粒细胞中依次出现的初级和次级颗粒均被发现是相对于高尔基体的反式成分形成的。
