Soler P, Moreau A, Basset F, Hance A J
INSERM U.82, CHU Xavier Bichat, Paris, France.
Am Rev Respir Dis. 1989 May;139(5):1112-7. doi: 10.1164/ajrccm/139.5.1112.
To evaluate the effect of cigarette smoking on the number, distribution, and differentiated state of dendritic cells (DC) and Langerhans cells (LC) in the human lung, we have quantitated the number of these cells present in the bronchioles and alveolar parenchyma of lung tissue from nonsmokers and cigarette smokers using anti-CD1 monoclonal antibodies which react preferentially with DC (M241) and LC (T6). M241+ DC were found in the bronchiolar submucosa and alveolar parenchyma of nonsmokers; T6+ LC were present within the bronchiolar epithelium. Cigarette smoking was associated with a twofold increase in the total number of cells of DC/LC lineage and a 30-fold increase in the number of T6+ cells, many of which contained Birbeck granules (LC), present in the alveolar parenchyma. Most LC found in the parenchyma of smokers were observed in close association with areas of alveolar type II pneumocyte hyperplasia. Cigarette smoking did not change the number of differentiated state of cells of DC/LC lineage within the bronchioles. Both DC and LC are present in the human lung. Cigarette smoking has an important effect on the number, distribution, and differentiated state of these cells, which may explain why most adult patients who develop Langerhans cell granulomatosis are smokers.
为评估吸烟对人肺中树突状细胞（DC）和朗格汉斯细胞（LC）的数量、分布及分化状态的影响，我们使用优先与DC（M241）和LC（T6）反应的抗CD1单克隆抗体，对非吸烟者和吸烟者肺组织的细支气管和肺泡实质中这些细胞的数量进行了定量。在非吸烟者的细支气管黏膜下层和肺泡实质中发现了M241 + DC；T6 + LC存在于细支气管上皮内。吸烟与DC/LC谱系细胞总数增加两倍以及肺泡实质中T6 +细胞数量增加30倍相关，其中许多T6 +细胞含有伯贝克颗粒（LC）。在吸烟者实质中发现的大多数LC与II型肺泡上皮细胞增生区域密切相关。吸烟并未改变细支气管内DC/LC谱系细胞的分化状态数量。DC和LC均存在于人肺中。吸烟对这些细胞的数量、分布及分化状态有重要影响，这可能解释了为何大多数发生朗格汉斯细胞肉芽肿病的成年患者是吸烟者。
