肌动蛋白自我组装的pH依赖性。
pH dependence of actin self-assembly.
作者信息
Wang F, Sampogna R V, Ware B R
机构信息
Department of Chemistry, Syracuse University, New York 13244.
出版信息
Biophys J. 1989 Feb;55(2):293-8. doi: 10.1016/S0006-3495(89)82804-8.
Fluorescence enhancement and fluorescence photobleaching recovery have been utilized to examine actin self-assembly over the pH range 6.6-8.0. The kinetics of assembly are faster and the critical concentrations are lower at lower pH. Filament diffusion coefficients are not a function of pH, indicating that average filament lengths are not pH dependent. Although critical actin concentrations are a sensitive function of the concentrations of various cations in the medium, the relative pH dependences of critical concentrations are similar for all combinations of cations employed. The pH dependence of actin self-assembly is sufficiently great that it should be taken into account when comparing data from different reports and when relating in vitro measurements to cytoplasmic mechanisms.
荧光增强和荧光漂白恢复已被用于研究在pH值6.6 - 8.0范围内的肌动蛋白自组装。在较低pH值下，组装动力学更快，临界浓度更低。丝状体扩散系数不是pH值的函数，这表明平均丝状体长度与pH值无关。尽管临界肌动蛋白浓度是培养基中各种阳离子浓度的敏感函数，但对于所使用的所有阳离子组合，临界浓度的相对pH依赖性是相似的。肌动蛋白自组装的pH依赖性足够大，以至于在比较不同报告的数据以及将体外测量与细胞质机制相关联时都应予以考虑。
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