血管内皮生长因子在牙源性角化囊性瘤、含牙囊肿和根端囊肿中的免疫组化表达：一项对比研究。
Immunohistochemical expression of vascular endothelial growth factor in keratocystic odontogenic tumor, dentigerous cyst, and radicular cyst: A comparative study.
作者信息
Khajuria Nidhi, Metgud Rashmi, Naik Smitha, Lerra Sahul, Tiwari Priya, Katakwar Payal, Tak Anirudh
机构信息
Department of Oral Pathology, Institute of Dental Sciences, Sehora, Jammu, Jammu and Kashmir, India.
Department of Oral Pathology, Pacific Dental College and Hospital, Udaipur, Rajasthan, India.
出版信息
Indian J Dent. 2016 Jan-Mar;7(1):17-22. doi: 10.4103/0975-962X.179378.
BACKGROUND
Cyst and tumors arise from tissue remains of odontogenesis, these interactions have been considered to play an important role in the tumorigenesis of odontogenic lesions. The connective tissue stroma has an essential role in the preservation of epithelial tissues and minor alterations in the epithelium are followed by corresponding changes in the stroma, such as angiogenesis. Vascular endothelial growth factor (VEGF) is considered the first factor which maintains its position as the most critical driver of vascular formation and is required to initiate the formation of immature vessels, with this aim, present study was executed to evaluate VEGF expression in kertocystic odontogenic tumor, dentigerous cyst and radicular cyst (RC).
MATERIALS AND METHODS
A retrospective study was carried out comprising a total of 31 cases; 13 cases of keratocystic odontogenic tumor (KCOT), nine cases of dentigerous cyst (DC) and nine cases of RC. The sections were stained immunohistochemically with VEGF antibody and were evaluated for the presence and intensity of the immuno reactive cells. Statistical analysis was carried out using Chi-square test to inter-compare the VEGF expression between KCOT, DC, and RC.
RESULTS
VEGF expression in the epithelium and connective tissue was significantly higher in KCOT compared to dentigerous and RC. One case of KCOT with carcinomatous change also revealed positive results for the VEGF expression in the dysplastic epithelium, tumor islands, and connective tissue. The significant difference was observed on inter-comparison of the VEGF expression in the connective tissue of KCOT and DC, whereas no significant difference was observed in the VEGF expression in the connective tissue of KCOT and DC.
CONCLUSION
The present study data supports the literature finding that angiogenesis can be important in the progression and enlargement of odontogenic cysts similarly to what occurs in neoplastic conditions and further it can be concluded that the higher positivity for VEGF of KCOT could help to explain in part the aggressive biological behavior of the lesion. The stroma of KCOT could be regarded not only as a structural support of the cyst wall but also as playing a part in the neoplastic behavior of cyst.
背景
囊肿和肿瘤起源于牙源性组织残余，这些相互作用被认为在牙源性病变的肿瘤发生中起重要作用。结缔组织基质在维持上皮组织方面具有重要作用，上皮组织的微小改变会伴随基质的相应变化，如血管生成。血管内皮生长因子（VEGF）被认为是维持其作为血管形成最关键驱动因子地位的首要因子，是启动未成熟血管形成所必需的。为此，本研究旨在评估VEGF在牙源性角化囊性瘤、含牙囊肿和根端囊肿（RC）中的表达。
材料与方法
进行一项回顾性研究，共纳入31例病例；13例牙源性角化囊性瘤（KCOT）、9例含牙囊肿（DC）和9例根端囊肿。切片用VEGF抗体进行免疫组织化学染色，并评估免疫反应细胞的存在情况和强度。采用卡方检验进行统计学分析，以比较KCOT、DC和RC之间的VEGF表达。
结果
与含牙囊肿和根端囊肿相比，KCOT上皮和结缔组织中的VEGF表达明显更高。1例发生癌变的KCOT病例在发育异常上皮、肿瘤岛和结缔组织中的VEGF表达也呈阳性结果。在比较KCOT和DC结缔组织中的VEGF表达时观察到显著差异，而在KCOT和根端囊肿结缔组织中的VEGF表达中未观察到显著差异。
结论
本研究数据支持文献发现，即血管生成在牙源性囊肿的进展和扩大中可能很重要，类似于肿瘤性疾病中的情况，并且可以进一步得出结论，KCOT中VEGF的较高阳性率可能有助于部分解释该病变的侵袭性生物学行为。KCOT的基质不仅可被视为囊肿壁的结构支撑，还在囊肿的肿瘤行为中发挥作用。
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