使用抗人血小板内皮细胞黏附分子抗体对牙源性肿瘤和囊肿中的血小板内皮细胞黏附分子进行免疫组织化学评估。
Immunohistochemical evaluation of podoplanin in odontogenic tumours & cysts using anti-human podoplanin antibody.
作者信息
Singhal Namrata, Khanduri Nitin, Kurup Deepak, Gupta Brijesh, Mitra Pranjan, Chawla Roshani
机构信息
Dept. of Oral Path, Institute of Dental Sciences & Advance Studies, Gwalior, MP, India.
Dept. of Pedo, Institute of Dental Sciences & Advance Studies, Gwalior, MP, India.
出版信息
J Oral Biol Craniofac Res. 2017 May-Aug;7(2):95-100. doi: 10.1016/j.jobcr.2017.05.001. Epub 2017 May 17.
BACKGROUND
Odontogenic Cysts & tumors originate through some aberration from the normal pattern of odontogenesis. Ameloblastoma is one of the most frequent intraosseous odontogenic tumors. However it is no longer appropriate to use the diagnosis of ameloblastoma without specifying the type. Varied-clinical entities of ameloblastoma differ in their biologic behaviour. Odontogenic cysts like dentigerous and radicular cysts are less aggressive in nature than odontogenic tumors. Recently, commonly used as a lymphatic endothelial marker in cancers has recently been found to play a possible role in odontogenic tumorigenesis also. Therefore the purpose of this study was to immunohistochemically analyse the expression of podoplanin in ameloblastomas, KCOTs, dentigerous cysts, radicular cysts & dental follicles.
METHODS
Paraffin-embedded tissue specimens of 15 Ameloblastomas (7 follicular, 6 unicystic, 2 desmoplastic),10KCOTs, 5 dentigerous cysts, 5 radicular cysts & 5 dental follicles were immunohistochemically examined using antibody against podoplanin.
RESULTS
All ameloblastomas displayed podoplanin expression in ameloblast-like cells of the epithelial islands while the stellate-reticulum like cells exhibited no or weak immunostaining. Expression of podoplanin in KCOTs was strongly positive in the cells of the basal and suprabasal layers & odontogenic epithelial nests. Positive immunoreaction for podoplanin was observed in the inflammatory radicular cysts and inflamed dentigerous cyst only and negative or weak expression in the lining epithelium of uninflamed dentigerous cysts and dental follicles.
CONCLUSION
Our results suggest that podoplanin can be used as a potential proliferative marker to observe the aggressive behaviour of ameloblastomas and KCOTs.
背景
牙源性囊肿和肿瘤起源于牙胚发生正常模式的某些异常。成釉细胞瘤是最常见的骨内牙源性肿瘤之一。然而，不明确类型而使用成釉细胞瘤的诊断已不再合适。成釉细胞瘤的不同临床实体在生物学行为上有所不同。牙源性囊肿，如含牙囊肿和根囊肿，其性质比牙源性肿瘤的侵袭性小。最近发现，一种在癌症中常用作淋巴管内皮标志物的物质，在牙源性肿瘤发生中也可能起作用。因此，本研究的目的是通过免疫组织化学分析足板蛋白在成釉细胞瘤、牙源性钙化上皮瘤、含牙囊肿、根囊肿和牙囊中的表达。
方法
使用抗足板蛋白抗体对15例成釉细胞瘤（7例滤泡型、6例单囊型、2例促结缔组织增生型）、10例牙源性钙化上皮瘤、5例含牙囊肿、5例根囊肿和5例牙囊的石蜡包埋组织标本进行免疫组织化学检查。
结果
所有成釉细胞瘤在上皮岛的成釉样细胞中均显示足板蛋白表达，而星网状细胞样细胞则无免疫染色或免疫染色较弱。牙源性钙化上皮瘤中足板蛋白在基底层和基底上层细胞以及牙源性上皮巢中呈强阳性表达。仅在炎性根囊肿和炎性含牙囊肿中观察到足板蛋白的阳性免疫反应，而在非炎性含牙囊肿和牙囊的衬里上皮中呈阴性或弱表达。
结论
我们的结果表明，足板蛋白可作为一种潜在的增殖标志物，用于观察成釉细胞瘤和牙源性钙化上皮瘤的侵袭行为。
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