Nam Eunryel, Lee Won Chul, Takeuchi Shoji
Institute of Industrial Science, The University of Tokyo, 4-6-1 Komaba, Meguro-Ku, Tokyo, 153-8505, Japan.
Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Macromol Biosci. 2016 Jul;16(7):995-1000. doi: 10.1002/mabi.201600068. Epub 2016 May 2.
A collagen sheet with highly aligned collagen fibers is fabricated by continuous cyclic stretch. The rearrangement of the collagen fibers depends on the different process parameters of the cyclic stretch, including magnitude, frequency, and period of stretch. The collagen fibers are aligned perpendicularly to the direction of the stretch. Corneal stromal cells and smooth muscle cells cultivated on the highly aligned collagen sheet show alignment along the collagen fibers without the stretch during culture. Thus, the sheet can be a suitable scaffold for use in regenerative medicine.
通过连续循环拉伸制备出具有高度排列胶原纤维的胶原片。胶原纤维的重排取决于循环拉伸的不同工艺参数，包括拉伸幅度、频率和周期。胶原纤维垂直于拉伸方向排列。在高度排列的胶原片上培养的角膜基质细胞和平滑肌细胞在培养过程中无需拉伸即可沿胶原纤维排列。因此，该片可作为再生医学中合适的支架。
