Department of Biomedical Engineering, University of Connecticut Health Center, Farmington, CT, 06030, USA.
Department of Pathology, Brigham and Women's Hospital, Boston, MA, 02115, USA.
Adv Mater. 2022 Mar;34(12):e2105883. doi: 10.1002/adma.202105883. Epub 2022 Feb 3.
Skeletal muscles play important roles in critical body functions and their injury or disease can lead to limitation of mobility and loss of independence. Current treatments result in variable functional recovery, while reconstructive surgery, as the gold-standard approach, is limited due to donor shortage, donor-site morbidity, and limited functional recovery. Skeletal muscle tissue engineering (SMTE) has generated enthusiasm as an alternative solution for treatment of injured tissue and serves as a functional disease model. Recently, bioprinting has emerged as a promising tool for recapitulating the complex and highly organized architecture of skeletal muscles at clinically relevant sizes. Here, skeletal muscle physiology, muscle regeneration following injury, and current treatments following muscle loss are discussed, and then bioprinting strategies implemented for SMTE are critically reviewed. Subsequently, recent advancements that have led to improvement of bioprinting strategies to construct large muscle structures, boost myogenesis in vitro and in vivo, and enhance tissue integration are discussed. Bioinks for muscle bioprinting, as an essential part of any bioprinting strategy, are discussed, and their benefits, limitations, and areas to be improved are highlighted. Finally, the directions the field should expand to make bioprinting strategies more translational and overcome the clinical unmet needs are discussed.
骨骼肌在重要的身体功能中发挥着重要作用,其损伤或疾病可导致运动能力受限和丧失独立性。目前的治疗方法导致功能恢复程度不一,而作为金标准方法的重建手术因供体短缺、供体部位发病率和功能恢复有限而受到限制。骨骼肌组织工程(SMTE)作为治疗受损组织的替代方案引起了人们的兴趣,并可作为功能障碍疾病模型。最近,生物打印技术作为一种有前途的工具,已被用于重现骨骼肌在临床相关大小下的复杂和高度组织化结构。本文讨论了骨骼肌生理学、损伤后的肌肉再生以及肌肉丧失后的当前治疗方法,然后对用于 SMTE 的生物打印策略进行了批判性回顾。随后,讨论了最近的进展,这些进展使得生物打印策略在构建大肌肉结构、促进体外和体内成肌、增强组织整合方面得到了改进。生物墨水是任何生物打印策略的重要组成部分,讨论了它们的优点、局限性和需要改进的领域。最后,讨论了该领域应该扩展的方向,以使生物打印策略更具转化性并克服临床未满足的需求。