Horvat J C, Pecot-Dechavassine M, Mira J C, Davarpanah Y
Laboratoire Biologie-Vertébrés, Université Paris Sud, Orsay.
Brain Res Bull. 1989 Jan;22(1):103-14. doi: 10.1016/0361-9230(89)90134-2.
Peripheral nerve (PN) autografts were used in the adult rat to join the midcervical spinal cord to a nearby denervated skeletal muscle. Retrograde tracing, morphological and electrophysiological studies indicated the following: 1) a great number of neurons, located bilaterally, between C3 and C7 in most laminae of the grey matter, extended axons into the PN grafts, 2) a lesser number of neurons regenerated up to the reconnected muscle, but most of them were typical motoneurons, 3) neuromuscular junctions were formed in ectopic locations, around the tip of the grafted nerve, and at the sites of original endplates, 4) these junctions were functional and formed by axons that had regenerated into the PN bridges, as muscle contraction was obtained by electrical stimulation of the grafted nerves, 5) they were proved to be cholinergic since endplate potentials, evoked by stimulating the PN graft, were suppressed by curare. These results strongly suggest that spinal neurons, and especially motoneurons, are involved in the formation, through PN bridges, of new functional cholinergic connections with denervated skeletal muscles.
在成年大鼠中使用周围神经（PN）自体移植物将颈中段脊髓与附近去神经支配的骨骼肌相连。逆行追踪、形态学和电生理学研究表明：1）大量双侧位于C3至C7之间灰质大多数板层中的神经元，将轴突延伸至PN移植物中；2）较少数量的神经元再生至重新连接的肌肉，但其中大多数是典型的运动神经元；3）神经肌肉接头在异位位置形成，围绕移植神经的末端以及在原始终板部位；4）这些接头具有功能，由再生进入PN桥的轴突形成，因为通过电刺激移植神经可引起肌肉收缩；5）由于刺激PN移植物诱发的终板电位可被箭毒抑制，证明它们是胆碱能的。这些结果强烈表明，脊髓神经元，尤其是运动神经元，通过PN桥参与了与去神经支配的骨骼肌形成新的功能性胆碱能连接。
