Vašut Radim J, Pospíšková Markéta, Lukavský Jan, Weger Jan
Department of Biology, Faculty of Education, Palacky University Olomouc, 779 00 Olomouc, Czech Republic.
Department of Botany, Faculty of Science, Palacky University Olomouc, 783 71 Olomouc, Czech Republic.
Plants (Basel). 2024 Feb 26;13(5):639. doi: 10.3390/plants13050639.
The genus , comprising some 400-500 species, is important in various alluvial or wet habitats of the northern hemisphere. It is a promising crop for applications such as biomass production, biofuels, or environmental projects. Clear species delimitation is crucial in ecology, biotechnology, and horticulture. DArTseq markers, a genome-wide technique, were tested for species and hybrid identification. A total of 179 willow samples were analysed, including six species of subgen. and four species of subgen. , including those used in biomass crop production, representing important European taxa. Identification of species-specific markers, clustering analyses (principal coordinate analysis, neighbor-joining) and Bayesian methods (Structure) unambiguously identified putative hybrids. In addition to demonstrating the high efficiency of DArT-seq markers in identifying willow hybrids, we also opened-up new questions about hybridisation processes and systematics. We detected unidirectional hybridisation between and , forming backcross hybrids, and we rejected the hypothesis that does not occur naturally in Europe. Further, the isolated position of within the genus was confirmed.
该属包含约400 - 500个物种，在北半球各种冲积或湿润生境中具有重要意义。它是生物质生产、生物燃料或环境项目等应用的一种有前景的作物。明确的物种界定在生态学、生物技术和园艺学中至关重要。DArTseq标记是一种全基因组技术，用于物种和杂种鉴定测试。共分析了179个柳树样本，包括柳亚属的6个物种和钻天柳亚属的4个物种，其中包括用于生物质作物生产的样本，代表了欧洲重要的分类群。通过鉴定物种特异性标记、聚类分析（主坐标分析、邻接法）和贝叶斯方法（Structure）明确鉴定出了推定杂种。除了证明DArT - seq标记在鉴定柳树杂种方面的高效性外，我们还提出了关于杂交过程和系统发育的新问题。我们检测到柳属和钻天柳属之间存在单向杂交，形成了回交杂种，并且我们否定了钻天柳在欧洲不会自然出现的假设。此外，钻天柳属在该属内的孤立位置得到了确认。
