Smith A, van Haaften-Day C, Russell P
Oliver Latham Laboratory, Department of Health, N.S.W., North Ryde, Australia.
Cancer Genet Cytogenet. 1989 Mar;38(1):13-24. doi: 10.1016/0165-4608(89)90160-x.
Cytogenetic analysis of a human ovarian carcinoma cell line JoN was performed at passages 6, 43, and 89. At passage 6 there were pseudodiploid and pseudotetraploid cells containing up to 22 markers. Pseudodiploid cells predominated. Significant differences were seen in the later analyses. At passages 43 and 89, cells had a modal number of 44 and 88 chromosomes with four new reciprocal translocations and loss of all unidentified markers. There was retention of one identified marker throughout plus one that gradually disappeared. Karyotypes at passages 43 and 89 were identical, suggesting stability of rearrangements. These changes indicate continuing chromosomal rearrangements in culture, not necessarily creating more complexity.
对人卵巢癌细胞系JoN在第6代、第43代和第89代进行了细胞遗传学分析。在第6代时，存在假二倍体和假四倍体细胞，含有多达22个标记。假二倍体细胞占主导。在后续分析中观察到显著差异。在第43代和第89代时，细胞的众数染色体数分别为44条和88条，有4种新的相互易位，所有未识别的标记均丢失。整个过程中保留了一个已识别的标记，还有一个逐渐消失。第43代和第89代的核型相同，表明重排具有稳定性。这些变化表明培养过程中存在持续的染色体重排，但不一定会产生更多复杂性。
