Meyer J M, Felten D L, Weyhenmeyer J A
College of Medicine and Neural and Behavioral Biology Program, University of Illinois, Urbana 61801.
Clin Exp Hypertens A. 1989;11(1):103-17. doi: 10.3109/10641968909035294.
Levels of immunoreactive angiotensin II (ANG II) were measured by radioimmunoassay in microdissected nuclei from the brain of the spontaneously hypertensive rat (SHR) and its normotensive control, the Wistar Kyoto rat (WKY). The nuclei assayed included the paraventricular nucleus of the hypothalamus (PVH), locus coeruleus (LC), nucleus of the solitary tract (NTS), dorsal motor nucleus of the vagus nerve (DMN of X) and the A1 region of the medulla. Sections of cerebral and cerebellar cortex were used as controls. Levels of immunoreactive ANG II ranged from 0.24 nmoles/g protein to 0.93 nmoles/g protein, with SHR brain containing significantly higher levels than WKY brain in the PVH and NTS. Although LC and DMN of X in the SHR brain contained higher levels of immunoreactive ANG II than WKY brain, the difference was not significant. No significant difference was found in the A1 region between the two species, and no detectable ANG II immunoreactivity was found in cerebral or cerebellar cortex. Since each of the nuclei studied has been implicated in the neural control of cardiovascular function, the increased levels of immunoreactive ANG II in the SH rat brain nuclei indicates a possible role for ANG II in the pathophysiology of hypertension in the SHR model.
采用放射免疫分析法，对自发性高血压大鼠（SHR）及其血压正常的对照品系Wistar Kyoto大鼠（WKY）大脑经显微切割的细胞核中的免疫反应性血管紧张素II（ANG II）水平进行了测定。所检测的细胞核包括下丘脑室旁核（PVH）、蓝斑（LC）、孤束核（NTS）、迷走神经背运动核（X的DMN）以及延髓的A1区。大脑和小脑皮质切片用作对照。免疫反应性ANG II水平在0.24纳摩尔/克蛋白质至0.93纳摩尔/克蛋白质之间，SHR大脑中PVH和NTS的ANG II水平显著高于WKY大脑。尽管SHR大脑中的LC和X的DMN所含免疫反应性ANG II水平高于WKY大脑，但差异不显著。两种品系在A1区未发现显著差异，且在大脑或小脑皮质中未检测到ANG II免疫反应性。由于所研究的每个细胞核均与心血管功能的神经控制有关，因此SH大鼠脑细胞核中免疫反应性ANG II水平的升高表明ANG II在SHR模型高血压病理生理学中可能发挥作用。
