Meyer J M, Felten D L, Weyhenmeyer J A
Neuroscience Program, University of Illinois, Urbana 61801.
Exp Neurol. 1990 Feb;107(2):164-9. doi: 10.1016/0014-4886(90)90154-k.
Levels of immunoreactive angiotensin II (ANG II) were measured in specific microdissected nuclei from the brains of newborn (NB; less than 1 week of age), 4-, 8-, and 12-week-old spontaneously hypertensive rats (SHR) and their age-matched normotensive controls, Wistar Kyoto (WKY) rats, using a sensitive radioimmunoassay. The structures investigated included the paraventricular nucleus of the hypothalamus (PVH), the nucleus of the solitary tract (NTS), the dorsal motor nucleus of the vagus (DMN of X), the locus coeruleus (LC), and the A1 region of the medulla. A section of cerebellar cortex was used as a control. Although ANG II was detected in each of the nuclei examined, there were no differences in the ANG II contents of any of these structures between young (NB and 4 week old) SH and WKY rats. However, by 8 weeks of age, the SHR had significantly higher ANG II levels in the PVH, NTS, and DMN of X than its normotensive control, and at 12 weeks of age, significantly higher ANG II levels were observed in the PVH, NTS, DMN of X, and LC of the SHR compared to those in the WKY. During the developmental period under investigation, both strains revealed increases in the ANG II content of all nuclei except for the LC, where the ANG II levels decreased with age. No detectable ANG II was found in the cerebellar cortex of either strain at any age.(ABSTRACT TRUNCATED AT 250 WORDS)
采用灵敏的放射免疫分析法，对新生（NB；小于1周龄）、4周龄、8周龄和12周龄的自发性高血压大鼠（SHR）及其年龄匹配的正常血压对照Wistar Kyoto（WKY）大鼠大脑特定显微切割核中的免疫反应性血管紧张素II（ANG II）水平进行了测定。研究的结构包括下丘脑室旁核（PVH）、孤束核（NTS）、迷走神经背运动核（X的DMN）、蓝斑（LC）和延髓A1区。取一段小脑皮质作为对照。虽然在所检查的每个核中都检测到了ANG II，但年轻（NB和4周龄）SHR和WKY大鼠的这些结构中ANG II含量没有差异。然而，到8周龄时，SHR的PVH、NTS和X的DMN中的ANG II水平明显高于其正常血压对照，在12周龄时，与WKY相比，SHR的PVH、NTS、X的DMN和LC中的ANG II水平明显更高。在研究的发育期间，两个品系除LC外的所有核中ANG II含量均增加，LC中的ANG II水平随年龄下降。在任何年龄，两个品系的小脑皮质中均未检测到可检测到的ANG II。（摘要截断于250字）
