Chaweeborisuit Phantip, Suriyonplengsaeng Chinnawut, Suphamungmee Worawit, Sobhon Prasert, Meemon Krai
Z Naturforsch C J Biosci. 2016;71(5-6):121-31. doi: 10.1515/znc-2015-0222.
Plumbagin, (5-hydroxy-2-methyl-1,4-naphthoquinone), a natural substance found in the roots of plant species in the genus Plumbago, has been used as a traditional medicine against many diseases. In this study, Caenorhabditis elegans was used as a model for testing the anthelmintic effect of plumbagin. The compound exhibited a nematicidal effect against all stages of C. elegans: L4 was least susceptible, while L1 was most susceptible to plumbagin with an LC(50) of 220 and 156 μM, respectively. Plumbagin inhibited C. elegans development from L1 to adult stages with an IC(50) of 235 μM, and body length was also reduced at concentrations of 25 and 50 μg/ml. Brood sizes decreased from 203±6 to 43±6 and 18±3 eggs per hatch in plumbagin-treated worms at 10, 25, 50 μg/ml, respectively. Furthermore, plumbagin was lethal to strains resistant to the nematicides levamisole, albendazole, and ivermectin, indicating that it possesses a strong and unique nematicidal action. Plumbagin decreased the number of mitochondria in hypodermal and intestinal cells and body wall muscles and damaged the ultrastructure of these tissues. Taken together, plumbagin may be a new drug against parasitic nematodes.
白花丹素（5-羟基-2-甲基-1,4-萘醌）是一种存在于白花丹属植物根部的天然物质，一直被用作治疗多种疾病的传统药物。在本研究中，秀丽隐杆线虫被用作测试白花丹素驱虫效果的模型。该化合物对秀丽隐杆线虫的各个阶段均表现出杀线虫作用：L4期最不敏感，而L1期对白花丹素最敏感，其半数致死浓度（LC50）分别为220和156μM。白花丹素抑制秀丽隐杆线虫从L1期发育至成虫期，半数抑制浓度（IC50）为235μM，在25和50μg/ml浓度下，线虫体长也会缩短。在10、25、50μg/ml的白花丹素处理组中，每只孵化线虫的产卵量分别从203±6降至43±6和18±3。此外，白花丹素对左旋咪唑、阿苯达唑和伊维菌素耐药的线虫株具有致死作用，表明其具有强大且独特的杀线虫作用。白花丹素减少了皮下、肠道细胞和体壁肌肉中线粒体的数量，并破坏了这些组织的超微结构。综上所述，白花丹素可能是一种抗寄生线虫的新药。
