氨基双膦酸盐扩增的Vγ9Vδ2 + T细胞的过继转移不能在人源化小鼠模型中控制HIV复制。
Adoptive transfer of aminobisphonate-expanded Vγ9Vδ2+ T cells does not control HIV replication in a humanized mouse model.
作者信息
Poonia Bhawna
机构信息
Institute of Human Virology, University of Maryland School of Medicine, Baltimore, MD, USA.
出版信息
Immunotherapy. 2016 May;8(5):521-6. doi: 10.2217/imt-2015-0003.
Abstract
AIM
Vγ9Vδ2 γδ T cells have effector potential against several cancers and infectious agents. Whether these cells control HIV replication was tested in humanized mouse model.
METHODS
NOD SCID gamma mice engrafted with human peripheral blood mononuclear cells (PBMCs) and infected with HIVBAL were treated with zoledronate-expanded Vγ9Vδ2 T cells either alone or in combination with an anti-HIV envelope antibody b12.
RESULTS
Severe depletion of CD4+CCR5+ T cells was observed in PBMCs of all infected mice. HIV plasma p24 levels were comparable in all infected groups with no decrease in plasma viremia achieved by adoptive transfer of cells.
CONCLUSION
Autologous transfer of ex vivo expanded Vγ9Vδ2 T cells may not be a successful treatment choice for controlling HIV replication in vivo.
摘要
目的
Vγ9Vδ2 γδ T细胞对多种癌症和感染因子具有效应潜能。在人源化小鼠模型中测试了这些细胞是否能控制HIV复制。
方法
将移植了人外周血单个核细胞（PBMC）并感染了HIVBAL的NOD SCID gamma小鼠，单独或与抗HIV包膜抗体b12联合使用唑来膦酸扩增的Vγ9Vδ2 T细胞进行治疗。
结果
在所有感染小鼠的PBMC中均观察到CD4+CCR5+ T细胞严重耗竭。所有感染组的HIV血浆p24水平相当，通过细胞过继转移未实现血浆病毒血症的降低。
结论
体外扩增的Vγ9Vδ2 T细胞的自体转移可能不是体内控制HIV复制的成功治疗选择。
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