Martini Federico, Poccia Fabrizio, Goletti Delia, Carrara Stefania, Vincenti Donatella, D'Offizi Gianpiero, Agrati Chiara, Ippolito Giuseppe, Colizzi Vittorio, Pucillo Leopoldo Paolo, Montesano Carla
National Institute of Infectious Diseases "Lazzaro Spallanzani" IRCCS, Rome, Italy.
J Infect Dis. 2002 Sep 15;186(6):847-50. doi: 10.1086/342410. Epub 2002 Aug 16.
T cells expressing Vgamma9Vdelta2 display lytic and proliferative responses against human immunodeficiency virus (HIV)-infected cells and release antiviral soluble factors. Chronic HIV-positive patients have deep changes in the composition and function of the circulating gammadelta T cell pool that are restored by highly active antiretroviral therapy (HAART). gammadelta T cells were analyzed during the rapid plasma HIV RNA rebound in HIV-infected patients undergoing structured treatment interruption (STI). A loss in circulating Vgamma9Vdelta2 T cells was observed, indicating that acute HIV replication may influence Vgamma9Vdelta2 homeostasis. These cells were lost among CD45RA(-)CD27(-) Vgamma9Vdelta2 T cell effectors, and a significant unresponsiveness, measured as antigen-driven interferon-gamma production, was observed during STI. After HAART resumption and consequent inhibition of HIV replication, Vgamma9Vdelta2 T cell reactivity was restored both quantitatively and functionally. These observations indicate that Vgamma9Vdelta2 T cells are activated early after active HIV replication but are rapidly lost when viremia is not controlled.
表达Vγ9Vδ2的T细胞对人类免疫缺陷病毒（HIV）感染的细胞表现出溶解和增殖反应，并释放抗病毒可溶性因子。慢性HIV阳性患者循环γδT细胞库的组成和功能发生了深刻变化，高效抗逆转录病毒疗法（HAART）可使其恢复。在接受结构化治疗中断（STI）的HIV感染患者血浆HIV RNA快速反弹期间，对γδT细胞进行了分析。观察到循环Vγ9Vδ2 T细胞减少，表明急性HIV复制可能影响Vγ9Vδ2的稳态。这些细胞在CD45RA（-）CD27（-）Vγ9Vδ2 T细胞效应器中减少，并且在STI期间观察到以抗原驱动的干扰素-γ产生衡量的显著无反应性。HAART恢复并随后抑制HIV复制后，Vγ9Vδ2 T细胞反应性在数量和功能上均得以恢复。这些观察结果表明，Vγ9Vδ2 T细胞在活跃的HIV复制后早期被激活，但在病毒血症未得到控制时会迅速丢失。
